Dynamic changes of CD59 and P2X7 expression in macrophages during myocardial infarction in mice

Gao Mingze; Meng Jiali; Gao Hui; Tian Xuemeng; Du Yunhui; Zhao Yannan; Zhang Jing

doi:10.3760/j.issn.1673-4777.2024.11.006

Abstract：Objective To investigate the dynamic changes of macrophage subpopulations and the expressions of CD59 and P2X7 during the process of myocardial infarction in mice.Methods A total of 36 adult male C57BL/6J mice were randomly divided into control group(6 mice without any treatment)and myocardial infarction group(30 mice).A mouse model of acute myocardial infarction was established by ligating the left anterior descending coronary artery,and 30 mice in the myocardial infarction group were randomly divided into groups A,B,C,D,and E,and the samples were collected at 1,3,5,7,and 14 d after myocardial infarction,respectively.The peripheral blood and heart tissues of mice were collected.The dynamic changes of macrophage subpopulations and the expressions of CD59 and P2X7 were detected by flow cytometry.Results The numbers of monocytes in peripheral blood on the 1 and 3 d after myocardial infarction were higher than those in the control group[(9.5±1.7),(10.3±3.7)x 104/ml vs(3.2±3.1)x 104/ml](all P＜0.05).The white blood cell counts and macrophage counts of heart tissue at 1,3 and 5 d after myocardial infarction were higher than those of the control group(all P＜0.05).The Ly6Chigh macrophage count in cardiac tissue peaked at 3 d after myocardial infarction,while the Ly6Clow subset reached a peak at 5 d after myocardial infarction,both of which were higher than those in the control group(all P＜0.05).The percentage of P2X7-CD59-cells in Ly6Chigh cells was higher than that in the control group on 1 d after myocardial infarction,while the proportion of P2X7+CD59+macrophages was lower than that in the control group(all P＜0.05).The percentages of P2X7+CD59+cells in Ly6Clow macrophages at 1,3,and 5 d after myocardial infarction were lower than those in the control group,while the proportion of P2X7-CD59+cells was higher than that in the control group(all P＜0.05).Conclusion At different stages of acute myocardial infarction in mice,the subpopulations of cardiac tissue macrophages show dynamic changes,accompanied by dynamic changes in the expressions of CD59 and P2X7.