Changes of serum microRNA-155 and NOD-like receptor protein 3 levels in patients with type 2 diabetes mellitus and their diagnostic values for carotid atherosclerosis

Zeng Yijia; Song Min; Yu Hong

doi:10.3760/j.issn.1673-4777.2024.09.015

Abstract：Objective To investigate the changes of serum microRNA-155(miR-155)and NOD-like receptor protein 3(NLRP3)levels and their diagnostic values for carotid atherosclerosis(CAS)in patients with type 2 diabetes mellitus(T2DM).Methods A total of 142 patients with T2DM admitted to West China Hospital,Sichuan University from January 2021 to January 2023 were selected.According to the presence or absence of CAS,the patients were divided into CAS group(72 cases)and non-CAS group(70 cases).The general data of the two groups of patients were collected and compared,and the serum levels of miR-155 and NLRP3 were detected and compared between the two groups.Pearson corr-elation was used to analyze the correlation between serum miR-155 and NLRP3 levels in T2DM patients.Multivariate Logistic regression was used to analyze the influencing factors of CAS in T2DM patients.Receiver operating characteristic curve was used to analyze the diagnostic value of serum miR-155 and NLRP3 levels for CAS in T2DM patients.Results The duration of T2DM in the CAS group was longer than that in the non-CAS group,and the proportion of smoking history,glycosylated hemoglobin,home-ostasis model assessment of insulin resistance index,miR-155 and NLRP3 were higher than those in the non-CAS group(all P＜0.05).Pearson correlation analysis showed that serum miR-155 levels were positively correlated with NLRP3 levels in T2DM patients(r=0.765,P＜0.001).Multivariate Logistic regression model showed that the prolonged duration of T2 DM,smoking history,glycosylated hemoglobin,homeostasis model assessment of insulin resistance index,miR-155 and NLRP3 were independent risk factors for CAS in T2DM patients(all P＜0.05).Receiver operating characteristic curve analysis showed that the area under the curve of serum miR-155 and NLRP3 levels combined in the diagnosis of CAS in T2DM patients was larger than those of serum miR-155 and NLRP3 levels alone(0.900 vs 0.791,0.799,Z=3.436,3.381;both P=0.001).Conclusions The increase of serum miR-155 and NLRP3 levels is closely related to the occurrence of CAS in T2DM patients.The combina-tion of serum miR-155 and NLRP3 levels has a high diagnostic value for CAS in T2DM patients.