The role of low methylation levels of the CD4+T lymphocyte perforin gene promoter in the progression of emphysema in smoking cessation rats and the intervention effect of S-adenosylmethionine
Lu Jiangtao
Zhang Yanmei
Cheng Guirong
Zhang Cheng
Abstract：Objective To investigate the relationship between the methylation levels of the CD4+T lymphocyte perforin gene promoter and the continuous progression of pulmonary emphysema in rats after smoking cessation,as well as the intervention effect of S-adenosylmethionine(SAM).Methods Twenty SD rats were randomly divided into 4 groups:normal control group,model group,4-week smoking cessation group,and 4-week smoking cessation with SAM intervention group,each comprising 5 rats,the last three groups rat emphysema model was established using cigarette smoke exposure method and taked corresponding measures.Right lower lung tissues were collected from each group for histopathological observation after staining with hematoxylin-eosin(HE),and mean linear intercept(MLI)and mean alveolar number(MAN)were measured.CD4+T lymphocytes were isolated from the spleens of rats in each group using immunomagnetic bead method,DNA was extracted,and the methylation level of the CD4+T lymphocyte perforin gene promoter region was detected.Results The model group,the 4-week smoking cessation group,and the 4-week smoking cessation with SAM intervention group all showed significant increases in MLI compared to the normal control group(all P＜0.05).Specifically,the 4-week smoking cessation group showed greater increases compared to the model group and the 4-week smoking cessation with SAM intervention group(both P＜0.05).Moreover,the model group,the 4-week smoking cessation group,and the 4-week smoking cessation with SAM intervention group demonstrated decrease in MAN compared to the normal control group(all P＜0.05),with the 4-week smoking cessation group showing a greater decrease compared to the model group(P＜0.05).Additionally,the methylation levels of CD4+T lymphocyte perforin gene promoter in the model group and the 4-week smoking cessation group were lower than those in the normal control group and the 4-week smoking cessation with SAM intervention group[(93.9±3.1)％and(93.1±1.8)％vs(97.1±1.1)％and(96.4±1.5)％](all P＜0.05).Conclusions The persistent low methylation levels observed in the perforin gene promoter of CD4+T lymphocytes in rats following smoking cessation may contribute to the ongoing deterioration of emphysema in this population.However,following intervention with SAM,the deterioration of pulmonary emphysema in smoking cessation rats could be reversed.
Keywords：EmphysemaSmoking cessation ratsCD4+T lymphocyte perforin gene promoter methyla-tion levels
Publication Date：2024-09-08
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)
Pages：4( 1334-1337 )