Telmisartan inhibits the proliferation of scirrhous gastric cancer cells through the phosphatidylinositol 3-kinase/protein kinase B/mammalian target of rapamycin signaling pathway
Bai Xihui
Liu Shiyu
Sun Yuanyuan
Abstract：Objective To investigate the inhibitory effect of telmisartan(TEL)on the proliferation of scirrhous gastric cancer(SGC)cells and to explore its mechanism.Methods Routine culture of gastric cancer cells MKN1 and SGC cells HSC45,Cells Count Kit-8 experiment to detect the effect of TEL on the proliferation ability of MKN1 and HSC45 cells.Flow cytometry was used to detect the effects of TEL on apoptosis and cell cycle of HSC45 cells.Western blotting was used to detect the effects of TEL on autophagy and phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT)/mammalian target of rapamycin(mTOR)signaling pathway related protein expression in HSC45 cells.HSC45 cells were treated with the PI3K/AKT/mTOR signaling pathway activator SC79 and TEL.The effects of TEL on HSC45 cell proliferation,apoptosis,autophagy,and cell cycle were detected,respectively,after activating the PI3 K/AKT/mTOR signaling pathway.Results TEL inhibited the proliferation ability of MKN1 and HSC45 cells in a concentration and time dependent manner(both P＜0.001),and had a significant inhibitory effect on HSC45 cell proliferation(P＜0.05).Compared with the control group,apoptosis rat in HSC45 cells,LC3 Ⅱ/Ⅰ protein expression cell cycle arrest in G0/G1 phase rate in TEL group were higher[(26.2±2.6)％vs(1.3±0.4)％,(1.02±0.09)vs(0.29±0.04),(53.4±3.4)％vs(38.1±2.9)％],and phosphorylated PI3K,AKT and mTOR expressions in TEL group were lower(all P＜0.05).Compared with the control group,proliferation rate,apoptotic rate,LC3 Ⅱ/Ⅰ protein expression,and cell cycle arrest in G0/G1 phase rate in TEL group and TEL+SC79 group were higher,while those in the TEL+SC79 group were lower than the TEL group(all P＜0.05).Conclusion TEL downregulates the PI3K/AKT/mTOR signaling pathway,and promotes SGC cell apoptosis,autophagy,and cycle arrest,thereby inhibiting SGC cell proliferation ability.
Keywords：TelmisartanScirrhous gastric cancerPhosphatidylinositol 3-kinase/protein kinase B/mammalian target of rapamycin signaling pathwayProliferation
Publication Date：2024-06-08
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)
Pages：5( 842-846 )