Neuroprotective effect of Yixiao decoction on diabetic peripheral neuropathy in mice

Li Bowu; Zhao Qiyao; Li Chunshu; Huang Jukai; Wen Yalu; Li Jiaoyue; Zhang Ye; Hu Chaoyue; He Zhongchen; Chen Hongdong; Wang Jianhua; Yang Xiaohui

doi:10.3760/j.issn.1673-4777.2024.05.020

Abstract：Objective To investigate the protective effect of Yixiao decoction on sciatic nerve in mice with diabetic peripheral neuropathy.Methods Twenty-four KKAy mice aged 8 weeks were completely random divided into model group,lipoic acid group and Yixiao decoction group,with 8 mice in each group.Eight C57BL/6J mice of the same age were used as the control group.The lipoic acid group was given lipoic acid 60 mg/(kg·d)by gavage,the Yixiao decoction group was given Yixiao decoction 7.7 g/(kg·d)by gavage,and the control and model groups were given distilled water by gavage.The drug was administered once daily for 12 weeks.The fasting blood glucose,body weight and neurological function of mice in each group were compared before and 4,8,12 weeks after intervention.Results The fasting blood glucose and body weight of the model group,lipoic acid group and Yixiao decoction group were higher than those of the control group at 8 and 12 weeks after intervention(all P＜0.05).The fasting blood glucose of the lipoic acid group was lower than that of the model group at 12 weeks after intervention.The fasting blood glucose and body weight of the Yixiao decoction group were lower than those of the model group at 8 and 12 weeks after intervention.The body weight at 12 weeks after intervention in the Yixiao decoction group was lower than that in the lipoic acid group(all P＜0.05).The mechanical pain thresholds of the model group,the lipoic acid group and the Yixiao decoction group were lower than those of the control group at before and 8,12 weeks after intervention(all P＜0.05).The tail flick times of before and 4,8,12 weeks after intervention in the model group,lipoic acid group and Yixiao decoction group were higher than those in the control group(all P＜0.001).The mechanical pain thresholds of the lipoic acid group and the Yixiao decoction group were higher than those of the model group at 8 and 12 weeks after intervention,and the tail flick times of the lipoic acid group and the Yixiao decoction group were lower than those of the model group at 4,8 and 12 weeks after intervention.The mechanical pain threshold at 12 weeks after intervention in the Yixiao decoction group was higher than that in the lipoic acid group[(2.54±0.24)g vs(1.95±0.28)g],and the tail flick times at 4,8 and 12 weeks after intervention were lower than those in the lipoic acid group(all P＜0.001).Conclusion Yixiao decoction can reduce sciatic nerve injury in mice with diabetic peripheral neuropathy,and its mechanism may be related to the regulation of Keap1-Nrf2-ARE signaling pathway and the downstream expression of superoxide dismutase and catalase.