Study on the mechanism of Yiqi Huoxue Jiedu decoction in the treatment of patients with platinum-resistant ovarian cancer

Wu Xiaoqing; Lu Wenping

doi:10.3760/j.issn.1673-4777.2024.05.003

Abstract：Objective To explore the mechanism of Yiqi Huoxue Jiedu decoction in the treatment of platinum-resistant ovarian cancer(PROC).Methods A total of 12 patients with PROC in the Department of Oncology,Guang'anmen Hospital,China Academy of Chinese Medical Sciences from September 2022 to March 2023 were selected.According to different treatment methods,the patients were divided into traditional Chinese medicine group and control group,with 6 cases in each group.The control group was treated with the regimen selected by the doctor,and the traditional Chinese medicine group was treated with the Yiqi Huoxue Jiedu decoction on the basis of the regimen selected by the doctor.Peripheral blood was collected,RNA was extracted from peripheral blood,reverse transcribed and cDNA was purified after qualified quality control,and transcriptome sequencing was performed using Illumina sequencing platform.DESeq2 software was used to screen differential long-chain non-coding RNA(lncRNA)and differential expression gene(DEG)between the two groups.The target genes of lncRNA were predicted by cis-regulation,and the results of predicted target genes and sequenced DEG were intercrossed to obtain important target genes and lncrnas regulating them.Gene ontology,Kyoto Encyclopedia of Genes and Genomes and protein interaction network(PPI)analyses were performed.Results Solute carrier family 3 member 2(SLC3A2)and BCL2-like protein 11(BCL2L11)were the important target genes regulated by Yiqi Huoxue Jiedu decoction,and they were regulated by lncRNA SNHG1,MIR4435-2HG and AC108463.3.The DEG regulated by Yiqi Huoxue Jiedu decoction was enriched in the functions of cell metabolism,cell autophagy and protein-DNA binding.Vascular endothelial growth factor receptor signaling pathway,apoptosis,autophagy,choline metabolism in cancer,and mammalian target of rapamycin signaling pathway were enriched.PPI showed that Yiqi Huoxue Jiedu decoction mainly regulated proteins with apoptosis,cell metabolism,DNA repair function,as well as classic oncogene sarcoma gene,mitogen-activated protein kinase 3,etc.Conclusion The targets of Yiqi Huoxue Jiedu decoction in the treatment of PROC patients are SLC3A2 and BCL2L11 regulated by lncRNA SNHG1,MIR4435-2HG and AC 108463.3,thereby regulating the biological behavior of cancer cells such as programmed cell death and the classical pathway of cancer.