Changes and clinical significance of serum long chain non-coding RNA H19 and neurite guidance factor-1 levels in newborns with hypoxic ischemic encephalopathy

Zhang Ran; Yuan Xunling; Li Jiaqiang

doi:10.3760/j.issn.1673-4777.2024.04.012

Abstract：Objective To investigate the serum levels of long chain non-coding RNA H1 9(lncRNA H19)and neurite guidance factor-1(NT-1)in newborns with hypoxic ischemic encephalopathy(HIE)and their clinical significance.Methods A total of 102 newborns with HIE admitted to the First Affiliated Hospital of Harbin Medical University from February 2019 to February 2021 were selected as HIE group.According to the severity of HIE,they were divided into mild group(30 cases),moderate group(41 cases)and severe group(31 cases).According to the prognosis of HIE newborns,they were divided into the good prognosis group(76 cases)and the poor prognosis group(26 cases).A total of 60 cases of umbilical fistula and urachal fistula treated by neonatal surgery during the same period were selected as the control group.Serum levels of lncRNA H19 and NT-1 were detected.Multivariate Logistic regression model was used to analyze the factors affecting the prognosis of newborns with HIE.The receiver operating characteristic(ROC)curve was used to analyze the value of each index in evaluating the prognosis of newborns with HIE.Results Serum levels of lncRNA H19 and NT-1 in the HIE group were lower than those in the control group[(1.17±0.24)vs(2.35±0.53),(312±71)ng/L vs(514±61)ng/L](both P＜0.001).Serum levels of lncRNA H19 and NT-1 in the mild,moderate,and severe groups showed a decreasing trend in sequence(all P＜0.05).Multivariate Logistic regression analysis showed that serum lncRNA H19 and NT-1 were risk factors for the poor prognosis in newborns with HIE(both P＜0.001).ROC curve analysis showed that the area under the curve of combined detection of lncRNA H19 and NT-1 was larger than that of lncRNA H19 and NT-1 alone(Z=4.912 and 4.763,both P＜0.001).Conclusions The levels of serum lncRNA H19 and NT-1 in newborns with HIE are reduced,which is related to the severity of HIE.The combination of the two has a high predictive value for the poor prognosis of children with HIE.