The expression of microRNA-452 and microRNA-762 in colon cancer tissue and their relationship with prognosis

Sun Weiluo; Liu Suying; Li Sijin; Zhou Longmei; He Peiyuan

doi:10.3760/j.issn.1673-4777.2024.03.019

Abstract：Objective To investigate the expression of microRNA(miR)-452 and miR-762 in colon cancer tissue and their relationship with patients'prognosis.Methods Ninety patients with colon cancer admitted to Affiliated Hospital of Chengde Medical University from April 2019 to April 2020 were collected as research subjects.Tumor tissue and adjacent tissue were taken during surgery,and the prognosis of the good prognosis group and the poor prognosis group were determined based on the 3-year follow-up.Real-time quantitative polymerase chain reaction was applied to detect the expression of miR-452 and miR-762 in tissues.Pearson correlation analysis was applied to analyze the correlation between miR-452 and miR-762 expression.Multivariate Logistic regression was applied to analyze the prognostic factors of colon cancer patients.Receiver operating characteristic curve was applied to analyze the prognostic value of miR-452 and miR-762 expression levels in tumor tissues of colon cancer patients.Results Compared with adjacent tissues,the expression levels of miR-452 and miR-762 in tumor tissues of colon cancer patients were obviously increased[(2.23±0.24)vs(1.00±0.00),(1.86±0.20)vs(1.01±0.13)](t=48.620,33.805,both P＜0.001).The expression levels of miR-452 and miR-762 were related to TNM staging and tumor differentiation(all P＜0.05).The incidence of poor prognosis was 27.8％(25/90)during 3-year follow-up.Compared with the good prognosis group,the expression levels of miR-452 and miR-762 in tumor tissue of patients in the poor prognosis group were obviously increased[(2.56±0.30)vs(2.10±0.22),(2.15±0.30)vs(1.75±0.16)](t=7.997,8.181,both P＜0.001).Pearson correlation analysis showed there was a positive correlation between the expression levels of miR-452 and miR-762 in tumor tissue of colon cancer patients(r=0.560,P＜0.001).Multivariate Logistic regression analysis showed that miR-452 and miR-762 were risk factors for poor prognosis in colon cancer patients(both P＜0.05).The area under the curve of the ROC of the combination of miR-452 and miR-762 in predicting the colon cancer was better than that of miR-4-52 alone(Z=2.745,P=0.006).Conclusion The expression of miR-452 and miR-762 is up-regulated in tumor tissues of colon cancer patients,and it is related to the prognosis of colon cancer patients and may be a biomarker for evaluating the prognosis of colon cancer.