Correlation between peripheral blood expression of circular RNA hsa_circRNA_000581 and phlegm syndrome of acute ischemic stroke
Hu Shuyan
Su Li
Liang Xueying
Zhu Lulu
Gu Lian
Lei Qinggui
Abstract：Objective To investigate the correlation between peripheral blood expression of circular RNA hsa_circRNA_000581 and phlegm syndrome of acute ischemic stroke(AIS).Methods A total of 171 AIS patients admitted to the First Affiliated Hospital of Guangxi University of Chinese Medicine from January 2016 to December 2020 were selected as the AIS group.A total of 182 Chinese Han normal people recruited from community health service centers in the same urban area as the source hospital of AIS group were selected as the control group.The relative expression level of hsa_circRNA_000581 in peripheral blood was measured by real-time fluorescence quantitative polymerase chain reaction.The diagnostic value of hsa_circRNA_000581 expression level for AIS was analyzed by receiver operating characteristic curve.The hsa_circRNA_000581 expression in the peripheral blood of the control group and different syndrome elements of traditional Chinese medicine were analyzed,and the blood routine and coagulation indexes of the high and low hsa_circRNA_000581 expression groups in patients with phlegm syndrome of AIS were compared.Results The expression level of hsa_circRNA_000581 in peripheral blood of the AIS group was significantly lower than that of the control group[4.43 × 10-4(2.90 × 10-4,7.14 × 10-4)vs 6.84 × 10-4(4.08 × 10-4,1.01 × 10-3)](P＜0.001).The area under the curve of peripheral blood hsa_circRNA_000581 expression level for diagnosing AIS was 0.657,with sensitivity of 69.1％and specificity of 62.0％.The expression level of hsa_circRNA_000581 in peripheral blood of AIS phlegm syndrome group(n=68)was significantly lower than that of control group(P=0.004).However,there was no statistically significant difference in the expression level of hsa_circRNA_000581 between the AIS wind syndrome group(n=55)and the control group(P=0.068).The levels of platelets count and fibrinogen in the hsa_circRNA_000581 low expression group(n=34)in AIS patients with phlegm syndrome were higher than those in the hsa_circRNA_000581 high expression group(n=34)(P=0.041,P=0.022).Conclusion Circular RNA hsa_circRNA_000581 may affect the occurrence of AIS phlegm syndrome by affecting coagulation function.
Keywords：Ischemic strokeSyndrome in traditional Chinese medicineCircular RNAhsa_circRNA_000581Coagulation function
Publication Date：2024-02-08
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)
Pages：5( 212-216 )