The diagnostic efficacy of real-time quantitative nucleic acid amplification of Mycobacterium tuberculosis/rifampicin-resistant test in pulmonary tuberculosis with bacterial negative cavity
He Yanfa
Yang Jiahui
Zhang Chunbo
Liu Tao
Qi Kelei
Abstract：Objective To evaluate the diagnostic efficacy of real-time quantitative nucleic acid amplification of Mycobacterium tuberculosis/rifampicin-resistant test(Xpert)in pulmonary tuberculosis with bacterial negative cavity.Methods From April 2021 to October 2022,data of 124 patients with pulmonary cavity disease admitted to Hebei Chest Hospital were collected,including 86 cases of pulmonary tuberculosis with bacterial negative cavity(observation group)and 38 cases of pulmonary cavity disease without tuberculosis(control group).The results of Xpert for surgical specimens and tuberculosis infection T cell spot test(T-SPOT)for peripheral blood were collected,and the diagnostic efficacy of the two on pulmonary tuberculosis with bacterial negative cavity was analyzed.Results The sensitivities of Xpert and T-SPOT in pulmonary tuberculosis with bacterial negative cavity were 68.6％and 75.6％,and the specificities were 97.4％and 68.4％,respectively.The sensitivity of Xpert was lower than that of T-SPOT(x2=5.832,P＜0.001),and the specificity was higher than that of T-SPOT(x2=21.469,P＜0.001).The positive rates of the two methods in the observation group were significantly higher than those in the control group[Xpert:68.6％(59/86)vs 2.6％(1/38);T-SPOT:75.6％(65/86)vs 31.6％(12/38)](both P＜0.001).Receiver operating characteristic curve analysis showed that the area under the curve was 0.830 for Xpert and 0.729 for T-SPOT in pulmonary tuberculosis with bacterial negative cavity,and the accuracy of the Xpert was higher than that of the T-SPOT(P＜0.001).Conclusions The detection of Xpert can provide reliable diagnostic support in the diagnosis of pulmonary tuberculosis with bacterial negative cavity,and realize the diagnosis and treatment as early as possible,so as to better control the patient's condition.
Keywords：Cavitary tuberculosisMycobacterium tuberculosisBacterial negative pulmonary tuberculosisReal-time quantitative nucleic acid amplification
Publication Date：2024-02-08
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)
Pages：5( 202-206 )