Correlation analysis of rs4741506 polymorphism of small nuclear RNA activation complex polypeptide 3 gene with ischemic stroke and its traditional Chinese medicine syndromes in Chinese Han population

Gu Lian; Liang Baoyun; Su Li; Liu Jing; Yang Yibing; Zeng Wenlu; Liang Yifan; Yan Yan

doi:10.3760/j.issn.1673-4777.2024.01.010

Abstract：Objective To investigate the correlation of small nuclear RNA activation complex polypeptide 3(SNAPC3)gene rs4741506 polymorphism with ischemic stroke(IS)and its traditional Chinese medical(TCM)syndromes in Chinese Han population.Methods Totally 774 IS patients admitted to Department of Encephalopathy,the First Affiliated Hospital of Guangxi University of Chinese Medicine from January 2016 to December 2019 were selected as the observation group,while during the same period 793 healthy examinees from Physical Examination Center or patients with mild trauma in Department of Orthopedics in the hospital were selected as the control group.IS patients were subjected to TCM syndrome differentiation,and SNAPC3 gene polymorphism site rs4741506 was genotyped.Four genetic models were established,and PLINK software and SPSS 21.0 software were used for genetic association analysis and correlation analysis between gene locus polymorphism and IS,TCM syndromes,and clinical indicators of patients.Results The genotype frequency distribution of rs4741506 locus in the observation group was significantly different from that in the control group(x2=6.366,P=0.041).In additive,dominant,and recessive models,there was no significant correlation between the rs4741506 polymorphism of the SNAPC3 gene and the risk of IS occurrence(all P＞0.05).The results of multivariate Logistic regression model analysis showed that after adjusting for age and gender,the rs4741506 polymorphism of the SNAPC3 gene was correlated with the risk of IS wind syndrome[recessive model:odds ratio(OR)=0.45,95％confidence interval(CI):0.22-0.92,P=0.029]and phlegm syndrome(recessive model:OR=0.39,95％CI:0.19-0.81,P=0.011);but there was no significant correlation with the risk of blood stasis syndrome(dominant model:OR=1.42,95％CI:1.00-2.01,P=0.051).Under the implicit model,the rs4741506 polymorphism of SNAPC3 gene was correlated with the diastolic blood pressure level in patients with IS phlegm syndrome after adjusting for age and gender(β=-10.93,95％CI:-20.64--1.22,P=0.028).General linear regression analysis showed that the rs4741506 polymorphism of SNAPC3 gene after adjusting for age and gender was correlated with serum apolipoprotein A1(additive model,dominant model),apolipoprotein B(recessive model),platelet count(additive model,dominant model)and thrombin time(dominant model)in patients with IS phlegm syndrome(all P＜0.05).Conclusion The SNAPC3 gene rs4741506 polymorphism may influence the development of wind syndrome and phlegm syndrome in IS.