Investigating the synergistic protective effect of levosimendan combined with shallow hypothermia on myocardial ischemia-reperfusion injury in rats
Li Lulu
Meng Yanhai
Li Zemeng
Zhang Yanbo
Abstract：Objective To investigate the synergistic protective effect of levosimendan combined with shallow hypothermia on myocardial ischemia-reperfusion injury in rats.Methods Totally 20 SD rats were randomly divided into levosimendan combined with mild hypothermia treatment(LM)group,levosimendan treatment(L)group,mild hypothermia treatment(M)group and control(C)group,5 rats per group.The anterior descending branch of the left coronary artery of rats was ligated to make a model of myocardial ischemia-reperfusion injury.Rats in different groups were given the corresponding dose(12 µg/kg)of levosimendan or 0.9％sodium chloride injection,which was infused intravenously at 0.3 µg/(kg·min)for 2 h while reperfusion,and maintained at shallow hypothermia(33.0±0.5)℃ or normothermia(37.0±0.5)℃ intervention for 2 h.Hemodynamic indexes were observed and recorded.Myocardial injury markers lactate dehydrogenase(LDH)and creatine kinase isoenzyme(CK-MB)were detected at the end of reperfusion,followed by 2,3,5-triphenyltetrazolium chloride staining to observe the volume of myocardial infarction.Results After 2 h of reperfusion,the mean arterial pressure and systolic blood pressure in the LM group,L group and M group were higher than those in the C group[(87±8),(76±7),(74±4)mmHg vs(63±7)mmHg;(121±9),(101±10),(100±10)mmHg vs(86± 5)mmHg],and the levels in LM group were higher than those in the L group and M group(all P＜0.05).After 2 h of reperfusion,the levels of LDH and CK-MB in LM group,L group and M group were lower than those in C group[(1 290±194),(1 590±205),(1 718±174)U/L vs(2 135±209)U/L;(1 060±35),(1 356± 214),(1 631±201)U/L vs(2 029±261)U/L],and the levels of LDH and CK-MB in LM group were lower than those in L group and M group(all P＜0.05).The myocardial infarction volume ratio in LM group,L group,and M group was lower than that in C group[(25±5)％,(38±4)％,(41±5)％vs(63±9)％],and the myocardial infarction volume ratio in LM group was lower than that in L group and M group(all P＜0.05).Conclusion Levosimendan combined with mild hypothermia treatment attenuated myocardial ischemia-reperfusion injury in rats and achieved synergistic protective effect by improving hemodynamics and reducing myocardial injury and myocardial infarct size.
Keywords：Myocardial ischemia-reperfusion injuryLevosimendanShallow hypothermiaSynergistic protection
Publication Date：2023-12-08
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)
Pages：5( 1778-1782 )