Correlation among preoperative serum microRNA-638 expression, lipid metabolism and prognosis in patients with non-small cell lung cancer
Gao Xuefeng
Liu Kun
Yu Xuhui
Yu Li
Zhang Rui
Abstract：Objective To explore the correlation among preoperative serum miRNA-638(miR-638) expression, lipid metabolism and the prognosis after radical surgery in patients with non-small cell lung cancer (NSCLC). Methods From February 2013 to March 2014, 201 NSCLC patients(NSCLC group) undergoing radical surgery and 30 healthy volunteers(control group) were enrolled at the Third People's Hospital of Hubei Province. Serum contents of total cholesterol(TC), triglyceride, high density lipoprotien(HDL) and low density lipoprotien( LDL) were tested before surgery. Expression of miR-638 mRNA in serum was detected by reverse transcription polymerase chain reaction. All NSCLC patients were followed up until January 31st 2019 and the overall survival condition was analyzed by Kaplan-Meier curve and Log-rank test. Results Levels of serum TC, HDL and miR-638 mRNA in NSCLC group were significantly lower than those in control group[(3. 4 ±0. 8)mmol/L vs (4. 5 ± 0. 8)mmol/L, (1. 15 ± 0. 34)mmol/L vs (1. 37 ± 0. 29)mmol/L, (0. 82 ± 0. 21) vs (1. 00 ± 0. 16)](all P<0. 01). Most patients with low HDL( <1. 04 mmol/L) showed low miR-638 expression[82. 2％(97/118) vs high miR-638 expression rate 17. 8％(21/118)](P<0. 001). Pearson analysis showed that miR-638 expression was positively correlated with HDL(R2 =0. 479, P<0. 001). Patients over 60 years old and with pTNM stageⅢshowed lower miR-638 expression(P=0. 014, 0. 039). Kaplan-Meier curve analysis showed lower 5-year survival rate in low miR-638 expression[39. 0％(46/118)vs 68. 7％(57/83) in high miR-638 expression](Log-rankχ2 =20. 172, P<0. 001). In subgroup analysis there were lower 5-year survival rates in pTNM stageⅠ-Ⅱand stageⅢpatients with low miR-638 expression than those with high miR-638 expression(both P<0.05). Conclusions Serum miR-638 mRNA expression decreases in NSCLC patients and its serum level is positively correlated with HDL. Patients with low miR-638 expression have poor 5-year survival outcome, indicating that miR-638 may be a potential indicator for the prognosis.
Keywords：Non-small cell lung cancerMicroRNA-638Pathological featureLipid metabolismPrognosis
Publication Date：2020-04-08
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)
Pages：5( 550-554 )