Effect of microRNA-9 targeting HMBOX1 on the biological function of gastric cancer cells
Zhu Jun
Yu Hualiang
Gao Shan
Ding Xiangwu
Abstract：Objective To investigate the effect of microRNA-9 (miR-9) on the biological function of gastric cancer cells by regulating HMBOX1.Methods Gastric cancer cell line BGC-823 was divided into blank group,control group and miR-9 group.The control group and miR-9 group were transfected with 50 nmoL/L miR-9 NC and miR-9 mimics,respectively;the blank group was treated with equal volume of phosphate buffer.Expression of miR-9 mRNA was detected by real-time fluorescence quantitative polymerase chain reaction.Cell proliferation was detected by methylthiazoletetrazolium assay.Cell apoptosis was detected by flow cytometry.HMBOX1 expression was detected by western blotting.Above experiments were repeated three times to obtain the average value.Results At 24 h and 48 h after transfection,expression of miR-9 and cell apoptosis index in the miR-9 group were significantly higher than those in the blank group and control group [(46.45 ± 11.82) vs (1.00 ± 0.12),(1.03 ±0.13);(55.45 ±10.21) vs (1.02 ±0.22),(1.11 ±0.32);(42.6 ± 1.5)％ vs (11.6 ±2.1)％,(13.2 ± 1.2) ％;(48.3 ± 1.5) ％ vs (17.4 ± 2.4) ％,(15.3 ± 1.8) ％] (all P ＜ 0.05);cell proliferation index in the miR-9 group was significantly lower than that in the blank group and control group [(0.42 ± 0.13) vs (1.11 ±0.78),(1.07 ±0.51);(0.53 ±0.13) vs (1.17 ±0.22),(1.12 ±0.12)] (all P ＜0.05);expression of HMBOX1 in the miR-9 group was significantly lower than that in the blank group and control group (all P ＜ 0.05).Conclusion Over-expression of miR-9 can inhibit the expression of HMBOX1,thereby inhibiting the proliferation of gastric cancer cells and promote apoptosis.
Keywords：Gastric cancerMicroRNA-9HMBOX1Cell biology
Publication Date：2020-01-08
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)
Pages：4( 83-86 )