MicroRNA-186 up-regulates Dicer1 inhibiting breast cancer cell line MCF-7 invasion and metastasis

Wang Minglei; Gao Qiaoyan; Zhang Daoqiang

doi:10.3760/j.issn.1673-4777.2020.01.017

Abstract：Objective To investigate the effect of microRNA-186 (miR-186) on invasion and metastasis of breast cancer cell line MCF-7 by regulating Dicerl.Methods Forty specimens of breast cancer tissue and paracancerous normal tissue determined by surgical and pathological findings were collected from January 2018 to February 2019 in Weihai Central Hospital,Shandong Province.Expressions of miR-186 in breast cancer tissue,paracancerous normal tissue,human breast cancer cell line MCF-7 and human normal breast epithelial cell line MCF10A were detected by real-time fluorescence quantitative polymerase chain reaction.Invasion ability of MCF-7 cells affected by miR-186 was examined by Transwell assay and cell migration ability was examined by scratch assay.The relation between miR-186 and Dicer1 was verified by bioinformatics software prediction and dual-luciferase reporter gene assay.Expression of Dicer1 was detected by western blotting.Results Breast cancer tissue had lower expression of miR-186 than normal adjacent tissue[(0.38 ±0.11) vs (0.98 ±0.24)] (P ＜0.05).Human breast cancer cells MCF-7 had lower expression of miR-186 than normal breast epithelial cells MCF10A[(0.14 ± 0.05) vs (1.04 ± 0.27)] (P ＜ 0.05).The number of MCF-7-mimic-miR-186 cells passing through Matrigel matrix gel and the scratch area were significantly less than those of MCF-7 and MCF-7-mimic-NC (all P ＜ 0.05).According to the prediction of www.microRNA.org website,miR-186 and Dicerl had complementary binding sequences,and dual-luciferase reporter gene assay confirmed the targeted binding relation between them.Breast cancer cells MCF-7 showed lower expression of Dicer1 than normal breast epithelial cells MCF10A (P ＜ 0.05).MCF-7-mimc-mir-186 cells showed higher expression of Dicer1 than MCF-7 and MCF-7-mimc-NC (both P ＜ 0.05).Conclusion MiR-186 inhibits the invasion and migration of breast cancer cell line MCF-7 by up-regulating Dicer1 expression.