Changes of esophageal mucosal microbiota in rats with reflux esophagitis treated with Xianglian tablets

Jia Mengzhen; Gao Feng; Sun Yue; Chen Xue; Zhang Jie

doi:10.3760/j.issn.1673-4777.2019.12.020

Abstract：Objective To investigate the effect of intragastric administration of Xianglian tablets on esophageal mucosal microbiota in rats with reflux esophagitis(RE).Methods Twenty-four healthy male SD rats were randomly divided into sham operation group,distilled water group(RE control group) and Xianglian tablets group (RE observation group),with 8 rats in each group.RE rat model was prepared by partial pyloric ligation and complete anterior gastric ligation.One week after operation,the RE observation group was treated with intragastric administration of Xianglian tablets dissolved in distilled water,while the sham operation group and RE control group were treated with intragastric administration of distilled water.Two weeks after medication,2 samples of lower esophageal mucosa were separated in each rat for bacterial genomic DNA extraction and pathological observation.Esophageal mucosal genomic DNA was categorized using 16S rDNA gene amplification and Illumina sequencing techniques.Pathological changes of RE was observed by hematoxylin-eosin staining.Results Six rats survived in each group.Abundance values of lower esophageal mucosal microbiota at the genus level showed significant differences among the sham operation,RE control group and RE observation group:Peptostreptococcus (2.5 ±0.5)％,(1.7±0.7)％ vs (2.5 ±0.3)％ (P=0.040);Helicobacter (1.5±0.1)％,(0.9±0.4)％ vs (1.6±0.1)％ (P =0.003);Lactococcus (0.8 ±0.1)％,(0.4 ±0.2)％ vs (0.7 ±0.1)％ (P =0.018).Pathological grading results of mild,moderate,severe were 1,3,2 cases respectively in the RE control group.All 6 cases in the RE observation group were mild and there was a statistical difference in pathological grading between the two RE groups(P =0.015).Conclusion Esophageal mucosal micmbiota changes in RE rats after treating with Xianglian tablets;the proportion of beneficial bacteria increases and it promotes the therapeutic effect on RE,which may provide a new direction for the treatment.