Changes and clinical significance of peripheral blood lymphocyte subsets and natural killer cells in children with infectious mononucleosis

Ye Cuiyan; Su Baofeng

doi:10.3760/j.issn.1673-4777.2019.12.017

Abstract：Objective To investigate the changes and clinical significance of peripheral blood lymphocyte subsets and natural killer (NK) cells in children with infectious mononucleosis (IM).Methods From January 2016 to June 2018,48 children with IM(observation group) and 48 healthy children(control group) were enrolled at Nanjing Hospital Affiliated to Nanjing Medical University.The observation group was treated with intravenous administration of ganciclovir.Lymphocyte subsets and NK cells were analyzed between groups and between IM children with the percentage of atypical lymphocytes≥ 10％ or ＜ 10％.Results Before treatment,CD3+,CDs+ in the observation group were significantly higher and CD4+,CD4+/CD8+,CD19+,NK cells were lower than those in the control group(all P ＜ 0.05).After treatment,CD3+ and CD8+ significantly decreased[(75 ± 11) ％ vs (82 ± 12) ％,(25±4)％ vs (32 ± 13)％],CD4+,CD4+/CDs+,CD19+ and NK cells significantly increased[(36 ±9)％ vs (22±4)％,(1.43±0.38) vs (0.68±0.11),(12.6 ±4.6)％ vs (7.3 ±2.4)％,(11.8 ±3.9)％ vs (8.1 ±2.6)％] in the observation group,as compared with before treatment(all P ＜ 0.05).In the observation group,CD4+,CD4+/CD8+ were lower[(21 ±4)％ vs (26 ±6)％,(0.61 ±0.13) vs (0.93 ±0.27)] and CDs+ was higher[(34 ±5)％ vs (28 ±8)％] with the percentage of atypical lymphocytes≥10％ than with the percentage of atypical lymphocytes ＜ 10％ (all P ＜ 0.05),and there were no significant differences in CD3+,CD19+ and NK cells between them(P ＞ O.05).Conclusions IM can cause abnormalities of peripheral blood T,B lymphocyte subsets and NK cells,and affects the immune function of children.Detection of lymphocyte subsets and NK cells can provide evidence for the diagnosis and immunotherapy in children with IM.