A preliminary study on the relationship between severity of reflux esophagitis and changes of esophageal mucosal microbiome

Yu Ying; Zhang Jie; Gao Feng; Chen Xue; Sun Yamei

doi:10.3760/j.issn.1673-4777.2019.10.019

Abstract：Objective To explore the relationship between severity of reflux esophagitis ( RE ) and changes of esophageal mucosal microbiome. Methods From May to October 2017, 32 RE patients diagnosed by gastroscopy in Beijing Anzhen Hospital, Capital Medical University were divided into mild RE group( Los Angeles typing A and B, 21 cases) and severe RE group( Los Angeles typing C and D, 11 cases);17 healthy volunteers were recruited as control group. Distal esophageal mucosal genome DNA was extracted and categorized using 16S rDNA gene amplification and Illumina sequencing technique. Results There were significant differences ofγ-Proteobacteria, Prevotella, Helicobacter and Moraxella abundances among mild RE group, severe RE group and control group [9. 0(8. 5,9. 7)％ vs 9. 9(9. 0,11. 1)％ vs 8. 7(8. 3,9. 2)％, 0. 12(0. 06,0. 16)％ vs 0. 13(0. 09,0. 18)％ vs 0. 20(0. 14,0. 35)％, 0. 04(0. 02,0. 07)％ vs 0. 02(0. 02,0. 04)％ vs 0. 10(0. 03,0. 14)％, 0(0,0)％ vs 0(0,0)％ vs 0.002(0,0.019)％](P = 0.049, 0.001, 0.034, 0.013). Gastroesophageal Reflux Disease Questionaire(GERDQ) score in severe RE group was significantly higher than that in mild RE group[8(8,11) vs 4(2,8)](Z= -2. 876,P=0. 004). Spearman analysis showed no correlation of GERDQ score with Firmicutes, Actinobacteria, Bacteroidetes and Proteobacteria; the correlation coefficients were 0. 213 ( P =0. 242 ) , -0. 082 ( P=0. 657 ) , 0. 068 ( P =0. 712 ) and 0. 204 ( P =0. 262 ) , respectively. Conclusion The composition of bacterial flora in distal esophageal mucosa of RE patients show obvious changes compared with healthy people, which may provide a new direction for the prevention and treatment of RE.