Effects of stromal cell-derived factor-1 and CXC chemokine receptor-7 on β-arrestin regulating metastasis and invasion of lung cancer cells
Gou Zhibin
Zhu Jianyong
Liu Haijuan
Zhang Libo
He Min
Ye Qing
Fan Rongmei
Li Junmin
Guo Guangyun
Abstract：Objective To explore the effects of stromal cell-derived factor-1 (SDF-1) and CXC chemokine receptor-7 (CXCR7) on metastasis and invasion of lung cancer cells regulated by β-arrestin.Methods Lung cancer cell line A549 were transfected by CXCR7 (CXCR7 group) and empty plasmid(transfection control group);A549 cells with no treatment were blank control group.Cell proliferation was detected by MTT assay;apoptosis was detected by flow cytometry;metastasis and invasion were detected by Transwell assay;SDF-1 and CXCR7 mRNA expression was detected by real-time quantitative polymerase chain reaction;protein expression was detected by western blotting.Results At 48 and 72 h after transfection,relative expression levels of SDF-1 and CXCR7 mRNA in CXCR7 group were significantly lower than those in transfection control group and blank control group(all P ＜ 0.05);cell proliferation inhibition rate and apoptosis rate in CXCR7 group were significantly lower than those in transfection control group and blank control group [48 h:(3.2 ± 1.3) ％ vs (10.4 ± 3.3) ％,(11.9±3.3)％;(4.4±1.8)％ vs (16.8±2.2)％,(18.9±3.2)％;72 h:(2.1 ±1.1)％ vs (14.0±2.1)％,(13.8±1.8)％;(5.4±1.2)％ vs (18.3 ±3.7)％,(20.1 ±2.8)％] (all P ＜0.05).At48 h after transfection,cell metastasis rate and invasion rate,relative expression levels of SDF-1,CXCR7 and β-arrestin in CXCR7 group were significantly higher than those in transfection control group and blank control group (all P ＜0.05).Conclusion SDF-1/CXCR7 can increase β-arrestin expression and promote proliferation,metastasis and invasion,inhibit apoptosis of lung cancer cells.
Keywords：Lung cancerStromal cell-derived factor-1CXC chemokine receptor-7β-arrestin
Publication Date：2019-01-01
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)
Pages：4( 1169-1172 )