Differential expression of long non-coding RNA in atrial tissue of atrial fibrillation rats
Zheng Wuyang
Liu Huixia
Huang Zhengrong
Huang Honglang
Wan Xiaoqun
Xie Qiang
Li Weihua
Abstract：Objective To explore the differential expression of long non-coding RNA(LncRNA) in atrial tissue between normal rats and atrial fibrillation rats.Methods Forty wild-type adult male SD rats were randomly assigned into control group and atrial fibrillation group with equal amount.The atrial fibrillation group had intravenous injection of 0.1 mL/100 g atrial fibrillation-inducing agents and the control group had intravenous injection of 0.1 ml/100 g saline for 7 days.Atrial fibrillation was detected by electrocardiogram.LncRNA microarray was performed to screen the differential expression.Fluorescence quantitative polymerase chain reaction was performed to verify the microarray data.Results Compared with the control group,the atrial fibrillation group showed a different LncRNA profile,including 5 up-regulated LncRNAs(TMEM252,USP18,CXCL10,OTOF,IFI27) and 4 down-regulated LncRNAs (GRASP,LAMB3,PLAU,SEMG1).Expression levels of TMEM252,USP18,CXCL10,OTOF and IFI27 in the atrial fibrillation group were significantly higher than those in the control group [(4.32±0.54) vs (0.49±0.13),(16.42 ±3.46) vs (2.31 ±0.51),(7.83 ±1.32) vs (1.18±0.33),(25.97±5.13) vs (4.52 ±1.92),(10.09 ±2.12) vs (1.36 ±0.63)];expressions of GRASP,LAMB3,PLAU and SEMG1 in the atrial fibrillation group were significantly lower than those in the control group[(0.92 ±-0.33) vs (2.49 ±0.82),(1.03 ±0.41) vs (5.13 ±1.12),(2.86 ±0.42) vs (14.38 ±1.93),(1.63 ± 0.44) vs (10.42 ± 2.19)] (all P ＜ 0.05).Conclusion LncRNA expression profile shows significant change in atrial tissue of rats suffering from atrial fibrillation.
Keywords：Atrial fibrillationLong non-coding RNAMicroarray
Publication Date：2019-01-01
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)
Pages：3( 835-837 )