Effect of octanol on astrocytes and glial fibrillary acidic protein in rats after cerebral ischemia-reperfusion
Wu Liao
Sun Ying
Sun Bo
Ni Xiaoyu
Xu Xin
Zuo Peng
Ni Guihua
Abstract：Objective To observe the effect of octanol on astrocytes and the expression of glial fibrillary acidic protein (GFAP ) after cerebral ischemia-reperfusion in rats and to explore the possible protective mechanisms of octanol. Methods Forty-eight healthy adult male SD rats were randomly divided into sham operation group,model group,solvent control group and octanol group,with 12 rats in each group. Model group, solvent control group and octanol group were made models of middle cerebral artery ischemia(2 h) -reperfusion (24 h) injury by thread embolization. At 30 minutes before ischemia,octanol group was intraperitoneally injected 5 mmol /kg octanol solution; solvent control group was injected the same volume of 5％ dimethyl sulfoxide solution; sham operation group and model group had 0.9％ sodium chloride injection. Neurological deficit score was assessed before execution. Neuronal damage was observed by Nessler staining. GFAP expression in ischemic penumbra was detected by immunohistochemistry and western blotting. Results After reperfusion(24 h) ,neurological deficit score in model group was significantly higher than that in sham operation group[(2.8 ± 0.4) vs 0](P <0.05); there was no significant difference between solvent control group[(2.8 ± 0.4) ] and model group(P >0.05); the score in octanol group[(1.1 ± 0.4) ] was significantly lower than that in model group(P < 0.05). After reperfusion(24 h) ,the number of GFAP-positive cells in model group was significantly more than that in sham operation group (P < 0.05 ). There was no significant difference of the number of GFAP-positive cells between solvent control group and model group (P > 0.05). The number of GFAP-positive cells in octanol group was significantly less than that in model group (P < 0.05). After reperfusion(24 h) ,expression of GFAP protein decreased in sham operation group and increased in model group; there was no significant difference between solvent control group and model group; expression of GFAP protein in octanol group was significantly lower than that in model group. Conclusion Octanol can inhibit the activation of astrocytes and protect brain against ischemia-reperfusion injury.
Keywords：Ischemia-reperfusionOctanolAstrocyteGlial fibrillary acidic protein
Publication Date：2019-01-01
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)
Pages：4( 706-709 )