Aging-related long chain non-coding RNA profile in human myocardium and blood

Tian Jiayu; Cheng Zichao; Zhao Jinyang; Fan Qian

doi:10.3760/j.issn.1673-4777.2019.05.008

Abstract：Objective To investigate the expression of long chain non-coding RNA (lncRNA) and microRNA(mRNA) in human myocardium and blood samples; to analyze the potential biological functions and pathways of aging-related lncRNA enriched in human myocardium and blood samples. Methods Seven heart tissues from patients undergoing cardiac surgery and 38 blood samples from 19 pairs of grandfathers /grandsons were collected. LncRNA and mRNA were detected through microarray and the differential expression was analyzed between the elderly group(grandfathers) and young group(grandsons) to find targets. With Veen diagrams,the lncRNAs /mRNAs which were aging-related and enriched in blood and myocardium were found and the biological functions and pathways were predicted. Finally,the targets of aging-related lncRNAs /mRNAs in co-expression were postulated and described. Results Totally 75 434 lncRNAs and 20 666 mRNAs were detected in myocardium; 75 434 lncRNAs and 20 666 mRNAs were detected in blood; 2 835 lncRNAs and 959 mRNAs showed significant differential expression between elderly group and young group. Taking the intersection among 24 281 lncRNAs enriched in blood samples,23 980 lncRNAs enriched in myocardium and aging-related lncRNAs in blood; 720 myocardial aging-related lncRNAs were found. Among 5 554 mRNAs enriched in blood samples,4 520 mRNAs enriched in myocardium and 959 aging-related mRNAs in blood; 63 myocardial aging-related lncRNAs were found. Co-expression of lncRNA and mRNA showed potential biological function and regulation between them; the lncRNA with central regulation role that affects mRNA expression and the possible mechanisms could be found. Conclusion Microarray analyzing the expression profile of aging-related lncRNA provides valuable information of the potential biological functions and pathways in myocardial aging and senescence.