Relation between peripheral blood active circulating tumor cell and clinical characteristics of non-small cell lung cancer

Xiao Yao; Zhu Guangfa; Zhang Ying; Wu Chunting; Fang Fang; Liu Shuang

doi:10.3760/j.issn.1673-4777.2019.04.012

Abstract：Objective To explore the relation between peripheral blood active circulating tumor cell (CTC) and clinical characteristics of non-small cell lung cancer(NSCLC).Methods From June 2017 to August 2018,51 NSCLC patients confirmed by imaging and pathological results were recruited in Beijing Anzhen Hospital,Capital Medical University(observation group),including 38 cases of adenocarcinoma and 13 cases of squamous cell carcinoma;21 patients with benign lung disease were recruited as benign lung disease group;18 healthy people were recruited as healthy control group.Peripheral blood active CTC was detected in vitro using recombinant virus vectors.Relation between the expression of CTC and the clinical and pathological features of NSCLC was analyzed.Results There were 44,4 and 0 positive CTC cases in NSCLC group,benign lung disease group and healthy control group,respectively.Sensitivity and specificity of CTC detection were 86.3％ (44/51) and 89.7％ (35/39).Numbers of active CTC in 6 ml peripheral blood showed significant differences between clinical stage Ⅰ-]Ⅱ and Ⅲ-Ⅳ,with and without distant metastasis(M0 vs M1) [1 (0,6) vs 3(0,54),1 (0,2) vs 3(1,8)](Z =-2.041,-2.623;P =0.041,0.009).Logistic regression showed that peripheral blood CTC level was independently associated with high risk of distant metastasis (odds ratio =1.356,95％ confidence interval:1.015-1.811,P =0.040);the risk of distant metastasis in NSCLC patients with peripheral blood CTC more than 3/6 ml increased nearly five times (odds ratio =5.356,95％ confidence interval:1.341-21.400,P =0.018).Conclusion Peripheral blood CTC detection,as a simple and noninvasive method,has high specificity and sensitivity in predicting distant metastasis of NSCLC.