Distribution of small RNA Cdc42 GTPase and microRNA-363-5p in different grades of glioma
Zhou Langlang
Chen Shengli
Abstract：Objective To investigate the distribution of small RNA Cdc42 GTPase and microRNA (miRNA)-363-5p in different grades of glioma.Methods Glioma samples of human were fixed by paraformaldehyde for 24 h and immersed in sucrose solution until it sank to the bottom.Partial tissues were made frozen section to test the expression of Cdc42 GTPase by immunohistochemistry.The rest samples were stored in liquid nitrogen,quantitative real-time polymerase chain reaction was used to detect expressions of Cdc42 GTPase and miRNA-363-5p in different grades of tumor.Results Polymerase chain reaction showed that the expression of miRNA-363-5p gradually increased[(1.401 ± 0.515) × 10-6,(1.665 ± 0.686) × 10-6,(5.792 ± 0.000) × 10-6,(8.383 ± 2.858) × 10-6] and the expression of Cdc42 GTPase was down-regulated [(2.218 ± 0.694) × 10-2,(2.004 ± 0.236) × 10-2,(0.285 ± 0.004) × 10-2,(0.071 ± 0.007) × 10-2] with the increase of tumor grade;there were significant differences in multiple comparisons (all P ＜ 0.05).Conclusion Cdc42 GTPase may be the target gene of miRNA-363-5p and they changed in an opposite direction of trend;up-regulation of miRNA-363-5p and down-regulation of Cdc42 GTPase indicate high grade of glioma.
Keywords：GliomaCdc42 GTPaseMicroRNA-363-5pCell proliferationApoptosis
Publication Date：2019-01-01
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)
Pages：4( 374-377 )