Expression and significance of helper T cell 9 in peripheral blood of pediatric bronchiolitis
Zhang Xuejing
He Xiao
Li Yingying
Li Dezhi
Wu Fuling
Shi Tao
Gao Meng
Abstract：Objective To analyze the expression and significance of helper T cell 9 (Th9 cell) and interleukin-9(IL-9) in peripheral blood of children with bronchiolitis.Methods Eighty children with bronchiolitis admitted to Binzhou Medical University Hospital from November 2016 to April 2018 were divided into mild group (n =29),moderate group (n =27) and severe group (n =24).Twenty-five healthy children were enrolled as control group.Flow cytometry and enzyme linked immunosorbent assay were used to detect Th9 cell and IL-9 in peripheral blood at acute phase and convalescence.Results Th9 cell ratio and IL-9 level in severe group,moderate group and mild group at acute phase were significantly higher than those in control group [(0.66 ± 0.18)％,(0.54±0.11)％,(0.43 ±0.15)％ vs (0.28 ±0.08)％;(182 ±19),(104 ±14),(68 ± 10) ng/L vs (32 ± 8)ng/L];there were also significant differences among the three bronchiolitis groups (all P ＜ 0.05).During convalescence of bronchiolitis,IL-9 levels in severe group,moderate group and mild group [(35 ± 7),(32 ± 8),(33 ±8)ng/L] were significantly lower than those at acute phase (all P ＜ 0.05);there was no significant difference of IL-9 level among the three bronchiolitis groups and control group (P ＞ 0.05).Conclusions In the acute phase of bronchiolitis,expressions of Th9 cell and its cytokine IL-9 in peripheral blood significantly increase with the severity of disease.Th9 cell and IL-9 may play an important role in the pathogenesis of bronchiolitis.
Keywords：BronchiolitisHelper T cell 9Interleukin-9
Publication Date：2019-01-01
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)
Pages：4( 353-356 )