Relation among intestinal flora, expressions of brain-derived neurotrophic factor and tight junction protein ZO1 in patients with functional gastrointestinal disorder

Wang Mu; Dong Lina; Zhang Fang; Wang Junping; Li Caihua

doi:10.3760/j.issn.1673-4777.2018.07.020

Abstract：Objective To analyze the relation among intestinal flora,expressions of brain-derived neurotrophic factor(BDNF) and tight junction protein ZO1 in patients with functional gastrointestinal disorder.Methods Thirty-two patients with functional gastrointestinal disorder undergoing enteroscopy from August 2014 to March 2015 in Shanxi Provincial People's Hospital were enrolled.Specimens of feces and colonic mucosa were collected to extract DNA;high-throughput sequencing of the V3-V4 region of 16S rRNA was performed.Expressions of BDNF and ZO1 mRNA in feces and colonic mucosa were detected by the real-time fluorescence quantitative polymerase chain reaction.Bacterial flora distribution of feces and colonic mucosa was observed using bioinformatics method.The relation among flora distribution,expressions of BDNF and ZO1 was analyzed.Results Abundance count of bacteria at genus level showed significant differences among specimens of fecal flora and colonic mucosal flora.At species level,over a dozen of strains of bacteria were negatively related to BDNF expression;many strains of bacteria were positively related to ZO1 expression.Gardnerella vaginalis and Sutterella_sp._252 in feces,Bacteroides vulgatus,uncultured Ruminococcus sp.,uncultured bacterium adhufec236,bacterium Shr3 and uncultured bacterium adhufec77.25 in mucosa were correlated with both BDNF and ZO1 expression.Butyrateproducing bacterium_GM2/1 and Bifidobacterium_longum were negatively related to BDNF expression in feces but positively related to ZO1 expression in mucosa.Conclusion Some strains of bacteria are correlated with both BDNF and ZO1 in patients with functional gastrointestinal disorder;the strains provide a new choice for the treatment of functional gastrointestinal disorder.