Effects of FTY720 and gemcitabine on expression of oncogene mdig in non-small cell lung cancer cells
Li Fangzhi
Hou Chunyang
Abstract：Objective To analyze the effects of FTY720 and gemcitabine on expression of oncogene mdig in non-small cell lung cancer cells.Methods Non-small cell lung cancer cell line A549 and H720 were separately treated with FTY720,gemcitabine and FTY720 + gemcitabine;cell proliferation,apoptosis and expression of mdig were analyzed.Results Inhibition of FTY720 on A549 and H520 cell proliferation showed dose and time dependence.Survival rates of A549 and H520 cells in FTY720 + gemcitabine group were significantly lower than those in FTY720 group and gemcitabine group[(21.8 ± 1.0)％ vs (60.1 ±7.3)％,(44.4 ±5.0)％;(23.3 ± 1.9)％ vs (43.6 ± 2.4) ％,(65.5 ± 2.9) ％] (P ＜ 0.05).Drug combination index of FTY720 + gemcitabine was lower than 1.Apoptosis rates of A549 and H520 cells in FTY720 + gemcitabine group were significantly higher than those in blank control group,FTY720 group and gemcitabine group(P ＜0.05).Expression of mdig in FTY720 + gemcitabine group,FTY720 group and gemcitabine group was significantly lower than that in blank control group [A549:(0.72 ±0.11)％,(0.62 ±0.09)％,(0.21 ±0.07)％ vs(0.86 ±0.12)％;H520:(0.69 ±0.08) ％,(0.63 ±0.10) ％,(0.18 ± 0.11) ％ vs (0.88 ± 0.21) ％];expression of mdig in FTY720 + gemcitabine group was significantly lower than that in FTY720 group and gemcitabine group (P ＜ 0.05).Conclusion FTY720 and gemcitabine can inhibit proliferation and promote apoptosis of non-small cell lung cancer cells and down-regulate mdig expression.
Keywords：Non-small cell lung cancerFTY720GemcitabineMdig
Publication Date：2018-01-01
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)
Pages：4( 854-857 )