Effect of polysaccharides extracted from vitis vinifera L.on learning and memory abilities and oxidative stress in rats with Alzheimer's disease
Li Yue
Ma Lijuan
Xiao Hui
Tian Tian
Yuan Yujuan
Yuan Fang
Abstract：Objective To investigate the effect of polysaccharides extracted from vitis vinifera L.(VTP) on learning and memory abilities and oxidative stress in Alzheimer's disease(AD) rats.Methods Sixty male SD rats were randomly divided into 6 groups (n =10):normal control group,model control group,positive control group(polyphenidazole 0.5 mg/kg),low dose VTP(50 mg/kg) group,middle dose VTP(150 mg/kg) group and high dose VTP(300 mg/kg) group.Rats in model control group,positive control group,low,middle and high dose VTP groups were made AD models by hippocampal injection of amyloid-β (Aβ).The model control group had distilled water gavage and other groups had drug gavage for 15 d.Cognitive function was evaluated by the Y-maze test.Indicators of oxidative stress in serum and hippocampus were detected,including superoxide dismutase (SOD),glutathione peroxidase(GSH-Px),catalase(CAT) and malondialdehyde(MDA).Results Number of Y-maze test errors in model control group was significantly more,total reaction time was significantly longer and number of tests before meeting the standard was significantly more than those in normal control group [(6.2 ± 1.5) times vs (1.8 ± 0.8) times,(158 ± 19) s vs (86 ± 16) s,(92 ± 8) times vs (57 ± 8) times] (P ＜ 0.05).Number of test errors in positive control group,middle and high dose VTP groups [(2.2 ± 0.8),(2.7 ± 1.2),(1.8 ±0.8)times] was significantly less,total reaction time[(87 ± 15),(89 ± 14),(83 ± 16) s] was significantly shorter and number of tests before meeting the standard[(60 ± 9),(75 ± 8),(63 ± 10)times] was significantly less than those in model control group(P ＜ 0.05).Levels of SOD,GSH-Px and CAT in serum and hippocampus in model control group were significantly lower and MDA was significantly higher than those in normal control group (P ＜ 0.05).Levels of SOD,GSH-Px and CAT in serum and hippocampus in positive control group,middle and high dose VTP groups were significantly higher and MDA was significantly lower than those in model control group (P ＜0.05).Levels of SOD,GSH-Px and CAT in hippocampus in low dose VTP group were significantly higher and MDA was significantly lower than those in model control group (P ＜ 0.05).Conclusion VTP can antagonize the neurotoxicity of Aβ by affecting oxidative stress response to improve learning and memory abilities of AD rats.
Keywords：Alzheimer's diseasePolysaccharides from vitis vinifera L.Y-mazeOxidative stress
Publication Date：2018-01-01
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)
Pages：5( 705-709 )