Mechanism of calcium regulatory proteins in cardioprotection after sevoflurane postconditioning
Yu Yang
Zhou Chenghui
Li Lihuan
Abstract：Objective To discuss the mechanism of calcium regulatory proteins in cardiac protection after sevoflurane postconditioning (SevoPoC).Methods Isolated hearts were separated from 30 SD rats to make ischemia-reperfusion injury(I/RI) models and they were randomly divided into 3 groups(n =10):①Time control (TC) group had continuous perfusion of oxygenated KH solution for 120 min;②I/RI group had continuous perfusion of oxygenated KH solution for 30 min,stopped 30 min,and then reperfused for 60 min;③SevoPoC group had continuous perfusion of oxygenated KH solution for 30 min,stopped 30 min,and then had 3％ sevoflurane perfusion for 10 min and KH solution perfusion for 50 min.Left ventricular end-diastolic pressure(LVEDP),heart rate(HR),left ventricular developed pressure(LVDP),the maximum LVDP increase rate(+ dp/dt) and the maximum LVDP decrease rate were observed.Myocardial infarct size was measured.Cardiac troponin (cTnI) in coronary outflow fluid was tested.Expressions of L-type Ca2+ channels (LTCCs),ryanodine receptor 2 (RyR2) and Na +-Ca2+ exchanger isoform 1 (NCX1) were determined.Results LVEDP in SevoPoC group was significantly lower,RPP(HR × LVDP) and + dp/dt were significantly higher than those in I/RI group (P ＜ 0.05).Level of cTnI in SevoPoC group was significantly lower than that in I/RI group[(0.060 ±0.003)mg/L vs (0.110 ±0.004)mg/L](P ＜0.01).NCX1 expression level in I/RI group was significantly higher than that in TC group[(0.59 ±0.09) vs (0.35 ± 0.05)];NCX1 expression level in SevoPoC group (0.32 ± 0.06) was significantly lower than that in I/RI group(P ＜ 0.01).Expressions of LTCCs and RyR2 showed no significant differences among groups(P ＞ 0.05).Conclusion SevoPoC can promote cardiac function recovery,increase cTnI release in coronary outflow fluid and reduce infarction size;the cardioprotection effect may be associated with NCX1 deactivation.
Keywords：Ischemia-reperfusion injurySevoflurane postconditioningCalcium regulatory proteinsNa +/Ca2 + exchanger
Publication Date：2018-01-01
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)
Pages：5( 485-489 )