Effect of KISS-1 gene on proliferation and invasion of human lung squamous carcinoma cell line YTMLC-90
Zhao Bin
Wang Junran
Zheng Suqin
Abstract：Objective To investigate the effect of KISS-1 gene on proliferation and invasion of human lung squamous carcinoma cell line YTMLC-90.Methods The pEGFP-N2-KISS-1 plasmid carried with KISS-1 gene and empty plasmid were respectively transfected into human YTMLC-90 cells;YTMLC-90 cells without special treatment were as a blank control group.Expression of KISS-1 protein was detected by western blot method to determine whether the transfection was successful.Proliferation activity of YTMLC-90 cells was tested by the MTT assay;invasion ability was tested by Millicell chamber method.Results KISS-1 gene was successfully transfected into YTMLC-90 cells in pEGFP-N2-KISS-1 plasmid group.The proliferation activity in pEGFP-N2-KISS-1 plasmid group had no significant differences compared to that in empty plasmid group and blank control group(P ＞ 0.05).The number of cells that passed through polycarbonate membrane of Millicell chamber in pEGFP-N2-KISS-1 plasmid group was significantly less than that in empty plasmid group and blank control group[(35 ± 5) × 105 vs (82 ± 6) × 105,(84 ± 5) × 105] (P ＜ 0.05).Conclusion KISS-1 gene has no effect on proliferation but inhibits invasion of YTMLC-90 cells,suggesting it may inhibit the invasion of lung cancer.
Keywords：Lung neoplasmsYTMLC-90 cellKISS-1 geneGene transfection
Publication Date：2018-01-01
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)
Pages：3( 48-50 )