Effect of γ-secretase inhibitor on T-bet in peripheral blood mononuclear cells in children with respiratory syncytial virus bronchiolitis
Liu Liangxiao
Zhang Xuejing
Wu Fuling
Wang Haiying
Abstract：Objective To investigate the effect of γ-secretase inhibitor MW167 blocking Notch signaling pathway on T-bet in peripheral blood mononuclear cells(PBMC) in children with respiratory syncytial virus(RSV)bronchiolitis.Methods From September 2016 to March 2017,30 children with RSV bronchiolitis(bronchiolitis group) and 20 healthy children (normal control group) were enrolled at Binzhou Medical University Hospital.PBMC was separated in normal control group and cultivated with dimethyl sulfoxide(DMSO);PBMC separated in bronchiolitis group was cultivated with DMSO(RSV group) and MW167 + DMSO(MW167 group) respectively.The level of γ-interferon in culture supernatant fluid was tested by enzyme linked immunosorbent assay;expressions of γ-interferon and T-bet mRNA in PBMC were tested by real-time fluorescent polymerase chain reaction method.Results The plasma level of γ-interferon in RSV group was significantly lower than that in control group [(60 ± 10)mg/L vs (112 ± 30)mg/L] (P ＜ 0.05);relative expression quantities of γ-interferon and T-bet mRNA in PBMC in RSV group[(0.21 ± 0.05),(0.33 ± 0.15)] were significantly lower than those in control group (1.00) (P ＜0.01).The plasma level of γ-interferon in MW167 group[(99 ± 10)mg/L] was significantly higher than that in RSV group (P ＜ 0.05);relative expression quantities of γ-interferon and T-bet mRNA in PBMC in MW167 group [(0.81 ± 0.25),(1.00 ± 0.21)] were significantly higher than those in RSV group (P ＜ 0.01).Conclusion γγ-secretase inhibitor can increase expressions of γ-interferon and T-bet in PBMC in children with RSV bronchiolitis.
Keywords：Capillary bronchitisT-betRespiratory syncytial virusNotch signaling pathwayγ-secretinase inhibitor
Publication Date：2017-10-30
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)
Pages：4( 1495-1498 )