Effect of bupivacaine on alveolar fluid clearance in ex vivo human lungs
Cui Yong
Jiang Xuedong
Yu Tong
Ding Yan
Nie Hongguang
Abstract：Objective To explore the effect of bupivacaine on alveolar fluid clearance(AFC) in ex vivo human lungs.Methods Segmental bronchi separated from patients who had lobectomy were divided into amiloride group (5 cases),bupivacaine group (6 cases),amiloride plus bupivacaine group (6 cases) and control group (6 cases) by random number table to have corresponding drug interventions;the control group had 10 ml,5％ calf serum albumin prepared with 0.9％ sodium chloride injection,the other 3 groups were added corresponding medicines.Ex vivo AFC was measured by Coomassie brilliant blue method.Expression levels of epithelial sodium channel(ENaC)-α protein and mRNA in H441 cells were tested by western blot and real-time polymerase chain reaction(PCR).Results The value of AFC in amiloride group,bupivacaine group and bupivacaine plus amiloride group were significantly lower than that in control group [(6.6 ± 1.2) ％,(9.6 ± 0.9) ％,(7.9 ± 2.1) ％ vs (14.0 ± 0.7) ％] (P ＜ 0.05);there were no significant differences of AFC among amiloride group,bupivacaine group and bupivacaine plus amiloride group (P ＞ 0.05).Western blot and real-time PCR results showed that protein and mRNA expression levels of ENaC-α in H441 cells in bupivacaine group after 24,48 h of bupivacaine intervention were significantly lower than those in control group [(0.55 ± 0.09),(0.49 ± 0.09) vs (1.00 ± 0.00);(0.28 ± 0.06),(0.26 ± 0.04) vs (1.00 ± 0.00)] (P ＜ 0.01).Conclusion The inhibition of lung fluid clearance caused by application of bupivacaine may be associated with the decrease of amiloride-sensitive ENaC expression.
Keywords：BupivacaineAlveolar fluid clearanceEpithelial sodium channel
Publication Date：2017-01-01
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)
Pages：4( 1333-1336 )