Correlation between length of telomere and injury of neural function in cerebral ischemia-reperfusion mice
Fu Hao
Yang Xiaosa
Sun Shengkai
Chen Xiaochu
Tu Yue
Cheng Shixiang
Wang Zhihong
Abstract：Objective To explore the the correlation between length of telomere and injury of neural function in cerebral ischemia-reperfusion mice.Methods Fifty-six male C57BL/6 mice were randomly divided into middle cerebral artery occlusion(MCAO) group(24 mice divided into 3 groups equally),sham group(24 mice divided into 3 group equally) and blank control group(8 mice).The MCAO group had MACO models by sutureoccluded method(1 h) and reperfusion;the sham group was ligated external carotid artery and common carotid artery for 1 h;the blank control group had no special treatment.At different time points after reperfusion(24,48,72 h),neural function was evaluated by modified Neurological Severity Score (mNSS);genomic DNA was extracted from peripheral blood to detect length of telomere by real-time fluorescence quantitative polymerase chain reaction method.Results The mNSS in MCAO group 24,48,72 h after reperfusion were significantly higher than those in sham group(P ＜0.05).Length of telomere 72 h after reperfusion in MCAO group was significantly shorter than that in sham group and blank control group [(0.72 ± 0.24) vs (0.97 ± 0.12),(1.04 ± 0.27)] (P ＜ 0.05).Length of telomere was negatively correlated with mNSS 72 h after reperfusion in MCAO group (r =-0.713,P ＜0.05).Conclusion The length of telomere can be significantly shortened 72 h after MCAO reperfusion in mice;which is associated with severity of neural function injury.
Keywords：Ischemia-reperfusionTelomereModified Neurological Severity ScoreOxidative stress
Publication Date：2017-01-01
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)
Pages：5( 537-541 )