Effect of full-flow cardiopulmonary bypass on glomerular filtration rate in SD rats

Li Shuwen; Jin Mu; Ma Qing; Li Lihuan

doi:10.3760/cma.j.issn.1673-4777.2017.01.021

Abstract：Objective To establish acute kidney injury (AKI) model induced by full-flow cardiopulmonary bypass (CPB) in rats;to analyze the effect of CPB on glomerular filtration rate (GFR).Methods Totally 20 male SD rats were randomly and equally divided into CPB group and sham group.Operations in CPB group were as follows:anesthesia induction,endotracheal intubation and mechanical ventilation;puncturing and catheterizing at ventral caudal artery and right jugular vein as channels for CPB perfusion and drainage.CPB channel included a venous reservoir,a peristaltic pump,a flow probe and a membrane oxygenator.CPB flow was maintained at 160-180 ml/(kg· min),CPB temperature was controlled at 34-36 ℃;body temperature,blood pressure and blood gas were monitored during CPB;the operation lasted for 120 min.Blood creatinine,urine creatinine and GFR were tested before CPB and 2 d,7 d after operation.The sham group was treated with endotracheal intubation,arteriovenous puncture and catheterization,without CPB;blood creatinine,urine creatinine and GFR were tested at the same time as CPB group.Survival rates were recorded 7 d after CPB.Results All rats survived 7 d after CPB.Blood creatinine and urine creatinine in CPB group were significantly higher than those in sham group 2 d after operation,GFR in CPB group was significantly lower than that in sham group[(395 ± 154) μmol/L vs (188±70)μmol/L,(8.2 ±2.9)mmol/L vs (5.2 ±1.9)mmol/L,(0.92 ±0.26)ml/(min · 100 g) vs (1.19 ± 0.25) ml/(min · 100 g)] (P ＜ 0.05).In CPB group,blood creatinine 2 d after CPB was significantly higher than that before operation [(291 ± 68) μmol/L] (P ＜ 0.05);blood creatinine,urine creatinine and GFR recovered to preoperative levels 7 d after operation.Conclusion Full-flow CPB can cause significant decrease of GFR in SD rats.