Effect of Yiqi Huoxue recipe on coronary endothelial cell injury in myocardial infarction rats
Geng Tao
Yin Ling
Liang Xiaoyan
Abstract：Objective To explore the effect of Yiqi Huoxue recipe on coronary endothelial cell injury in myocardial infarction rats.Methods Left anterior descending coronary artery ligation method or tying loose knot method were used to make rat myocardial infarction model and sham operation model (sham operation group,20 cases);myocardial infarction rats were randomly divided into model control group,Tongxinluo group,perindopril group and Yiqi Huoxue recipe group,with 23 rats in each group.The sham operation and model control group were intragastricly administrated sterile distilled water at the first day of molding;the Tongxinluo group was intragastricly administrated Tongxinluo 0.25 g/kg;the perindopril group had perindopril 0.4 mg/kg and the Yiqi Huoxue recipe group had Yiqihuoxue recipe 25 g/kg.Serum levels of nitric oxide(NO),endothelin-1 (ET-1),prostaglandin I2 (PGI2),thromboxane A.2 (TXA2),soluble endothelial cell protein C receptor (EPCR) and soluble thrombomodulin(TM) 7,28 d after modeling were determined through enzyme linked immunosorbent assay;EPCR and TM mRNA in myocardial tissue were extracted through Trizol method and EPCR and TM mRNA expression were determined through polymerase chain reaction.Results Serum levels of ET-1,TXA2,TM,EPCR and TM mRNA,EPCR mRNA expressions in myocardial tissue in model control group were significantly higher than those in sham operation group [7 d after molding:(15.8 ± 1.7) ng/L vs (5.3 ± 1.2) ng/L,(27.1 ± 2.5) ng/L vs (10.7 ±0.8)ng/L,(99 ±8)ng/L vs (53 ± 10) ng/L,(351 ± 27)ng/L vs (221 ± 21) ng/L,(1.86 ±0.98) vs (1.21 ±0.23),(2.09 ±0.61) vs (1.53 ±0.11);28 d after molding:(15.6 ±2.3) ng/L vs (6.3 ± 1.7)ng/L,(21.7 ± 7.5) ng/L vs (9.6 ± 2.7) ng/L,(110 ± 11) ng/L vs (66 ± 8) ng/L,(399 ± 36) ng/L vs (205 ± 27) ng/L,(3.71 ± 1.22) vs (1.24 ± 0.32),(4.09 ± 1.36) vs (1.49 ± 0.29)] (P ＜ 0.01);serum NO and PGI2 levels in model control group were significantly lower than those in sham operation group [7 d after molding:(18.6 ±3.3)ng/L vs (30.2 ± 2.6) ng/L,(121 ± 19) ng/L vs (187 ± 20) ng/L;28 d after molding:(15.2 ± 3.0) ng/L vs (31.4 ± 0.0) ng/L,(183 ± 26) ng/L vs (220 ± 19) ng/L] (P ＜ 0.01).Serum levels of ET-1,TXA2,TM,EPCR and expression levels of TM mRNA,EPCR mRNA in myocardial tissue in Tongxinluo group,perindopril group and Yiqi Huoxue recipe group 7,28 d after moding were lower and serum NO,PGI2 levels were higher than those in model control group,the differences were significant(P ＜ 0.01).Conclusion Yiqi Huoxue recipe in myocardial infarction rat model can reduce endothelial cells injury,improve endothelial function,regulate microcirculation homeostasis and relieve myocardial ischemia.
Keywords：Myocardial infarctionYiqi Huoxue recipeCoronary microcirculationEndothelial injury
Publication Date：2017-01-30
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)
Pages：5( 23-27 )