Establishment of chronic obstructive pulmonary disease rat model with simple fumigation method
Sun Jialin
Suo Hong
Abstract：Objective To establish the chronic obstructive pulmonary disease (COPD) model in rats through simple fumigation method.Methods A total of 60 specific-pathogen-free Sprague Dawley rats were divided into blank group (10 cases) and COPD model group (50 cases) according to the random number table method.The blank group was normally fed,the COPD model group was treated with simple fumigation for 60 d to induce COPD.The general morphology and pathological morphology of rats were observed;levels of serum interleukin 10 (IL-10),tumor necrosis factor α (TNF-α) and granulocyte colony-stimulating factor (G-CSF) were analyzed.Results Rats in COPD model group appeared typical COPD symptoms of cough,expectoration and wheeze,some rats were observed transparent viscous liquid in mouths and noses;rats in blank group had no cough and expectoration.Serum TNF-oα level in COPD model group was significantly higher than that in blank group[(2.27 ±0.28)nmol/L vs (1.96 ± 0.06) nmol/L] (P ＜ 0.05).IL-10 and G-CSF levels had no significant differences between groups (P ＞0.05).Lung tissues of rats in COPD model group were observed typical pathological characteristics under microscope;rats in blank group showed normal lung tissue morphology.Conclusion Smoking is the most important pathogenic factor of COPD;COPD model can be successfully prepared through simple fumigation method in rats.
Keywords：Pulmonary disease,chronic obstructiveFumigation
Publication Date：2016-12-30
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)
Pages：3( 1778-1780 )