Effect of calcineurin on astrocyte injury induced by tumor necrosis factor-α
Gao Ling
Miao Jun
Abstract：Objective To explore the effect of calcineurin on astrocyte injury induced by tumor necrosis factor-α(TNF-α).Methods Neonatal Wistar rats born within 24 h were as experimental subjects;cerebral cortex tissues were sampled for astrocyte culture.Primary cultured astrocytes were randomly divided into control group,TNF-α group(adding TNF-α 100.00 μg/L),ciclosporin A(adding CsA)group(CsA 1 g/L) and TNF-α ± CsA group(adding TNF-α 100.00 μg/L and CsA 1 g/L).One hundred and twenty minutes after adding reagents,absorbance value,lactate dehydrogenase (LDH) release rate,apoptosis rate,necrosis rate were tested by methylthiazolyldiphenyl-tetrazolium bromide assay.Results In TNF-α group,absorbance value was significantly lower than that in control group(0.65 ± 0.21 vs 0.93 ± 0.13) (P ＜ 0.05);LDH release rate was significantly higher than that in control group[(38.5 ±0.8) ％ vs (20.3 ± 1.1) ％] (P ＜0.01);apoptosis rate and necrosis rate were significantly higher than those in control group[(17.4 ± 1.8) ％ vs (7.8 ± 1.2) ％,(35.7 ± 3.9) ％ vs (5.2 ±0.7) ％] (P ＜ 0.05).Differences of absorbance value,LDH release rate,apoptosis rate and necrosis rate between TNF-α group and TNF-α + CsA group were all not significant (P ＞ 0.05).Conclusion TNF-α induced astrocyte injury is achieved through non-calcineurin pathway.
Keywords：CalcineurinAstrocyteTumor necrosis factor-α
Publication Date：2016-01-01
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)
Pages：4( 1481-1484 )