Effect of artesunate on apoptosis of retinal cells in diabetes rats
Li Tieying
Guan Xiaohai
Xing Chaobin
Cao Fuyuan
Cheng Guang
Chen Jing
Abstract：Objective To investigate the effects of artesunate (ART) on apoptosis of retinal cells in diabetes rats.Methods Totally 18 healthy male Sprague Dawley rats were randomly divided into control group,model group and ART treated group.The rats in model group and ART treated group were given intraperitoneal injection of streptozotocin to establish diabetes model;3 months later,the rats in ART treated group were given intraperitoneal injection of ART [20 mg/(kg · d)],and the rats in control group and model group were given intraperitoneal injection of 0.9％ sodium chloride injection.Ten days later,the eyeballs were isolated,periodic acidSchiff (PAS) stain was used to observe the morphological changes of retina,quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to detect the mRNA levels of B-cell lymphoma 2 (Bcl-2) and Bcl-2-associated x (Bax).Results Pathologically changes of retinal morphology was observed in model group in PAS stain,which was significantly improved in ART treated group.In model group,the Bcl-2 mRNA level in retinal tissue was significantly lower than that in control group [(0.72 ± 0.05) vs (1.23 ± 0.43)],in ART treated group,it was significantly higher (2.46 ± 0.34) than that in model group and control group (P ＜ 0.05 or P ＜0.01);the Bax mRNA level in retinal tissue in ART treated group was significantly lower than that in model group and control group [(0.51 ±0.16) vs (1.13-±0.26),(0.98-±0.07)] (P ＜0.01),while was not significantly different between model group and control group (P ＞ 0.05).Conclusion Artesunate has inhibitory function on apoptosis of retinal cells in diabetes rats.
Keywords：Diabetic retinopathyArtesunateApoptosis
Publication Date：2016-01-01
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)
Pages：4( 536-539 )