Expression of Occludin protein and its influence on blood brain barrier after cardiopulmonary resuscitation in sudden cardiac arrest rats
Zhang Huili
Luo Xurui
Li Ailian
Zhang Jie
Abstract：Objective To investigate the expression of Occludin protein after cardiopulmonary resuscitation (CPR) in sudden cardiac arrest rats and its effect on blood brain barrier.Methods Totally 200 Sprague-Dawley male rats were randomly divided into control group (n =100) and CPR group (n =100).Only arteriovenous and endotracheal intubations were given in control group,without choking and CPR;sudden cardiac arrest model was established,then CPR was performed in CPR group.Fifty rats were sacrificed with decollation to collect the brain tissue 2,6,12,24 and 48 h after postoperative stable phase (10 min after operation) in control group and 2,6,12,24 and 48 h after return of spontaneous circulation (ROSC) in CPR group (n =10 at each time points);the Occludin expression in the brain tissue was measured by immunohistochemistry.Fifty rats were injected with 3 mg/kg Evans blue via caudal veins,then were sacrificed with decollation 2 h later to collect the brain tissue;the brain barrier permeability rates of Evans blue were measured by fluorescence spectrophotometer.Results The cell number of positive Occludin protein and Evans blue content in brain tissue were not significantly different between groups (P ＞ 0.05).Along with time,the cell number of positive Occludin protein in brain tissue firstly decreased then increased in CPR group,being significantly lower than that in control group at all the time points [(68.2± 1.0)/high power field vs (86.8 ± 1.3)/high power field,(42.1 ± 1.6)/high power field vs (85.1 ±2.7)/high power field,(18.1 ± 1.1)/high power field vs (84.9 ±2.8)/high power field,(37.8 ± 0.7)/high power field vs (82.1 ± 3.1)/high power field,(61.2 ± 1.0)/high power field vs (83.8 ± 2.5)/high power field] (P ＜ 0.05);along with time,the contents of Evans blue in brain tissue firstly increased then decreased in CPR group,being significantly higher than that in control group at all time points [(0.496 ± 0.042) μg/gvs (0.245 ±0.012) μg/g,(0.704±0.021) μg/g vs (0.236±0.016) μg/g,(0.898 ± 0.047) μg/g vs (0.239±0.011) μg/g,(0.874±0.035) μg/gvs (0.251 ±0.019) μg/g,(0.692± 0.036) μg/g vs (0.232 ±0.043) μg/g] (P ＜0.05).The cell number of positive Occludin proteins in CPR group was negatively correlated with Evans blue content (r =-0.862,P ＜ 0.01).Conclusion Occludin protein expression in brain is decreased and the close connecting structure is damaged after CPR in rats,leading to increased permeability of blood-brain barrier.
Keywords：Cardiac arrestCardiopulmonary resuscitationOccludin proteinsBlood-brain barrierRats
Publication Date：2016-04-30
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)
Pages：5( 506-510 )