Influence of aging on levels of reactive oxygen species and reactive nitrogen media in patients and rats with acute myocardial infarction
Fan Qian
Yang Xiaochun
Zheng Xuwei
Zhao Donghui
Li Fang
Liu Jinghua
Abstract：Objective To explore the influence of aging on levels of reactive oxygen species (ROS) and reactive nitrogen species (RNS) in patients and rats with acute myocardial infarction (AMI) by clinical research and animal experiments.Methods Totally 104 patients with AMI who underwent percutaneous coronary intervention (PCI) from September 2013 to March 2015 were selected,37 adult patients were finally enrolled and divided into adult group (＜65 years old,37 cases) and elderly group (≥65 years old,49 cases).The plasma levels of ROS [malondialdehyde (MDA),myeloperoxidase (MPO) and glutathione (GSH)] and RNS [inducible nitric oxide synthase (iNOS) activity,NOx and nitrotyrosine (ONOO-)] 24 h and 3 d after PCI were compared between groups.Because of the limitation of clinical research,a corresponding animal experiment was designed.Totally 72 Sprague Dawley rats were randomly divided into 6 groups (young rats 2 months old,middle-aged rats 10 months old,elderly rats 24 months):young control group,middle-aged control group,elderly control group,young ischemia/reperfusion (L/R) group,middle-aged I/R group,elderly I/R group (12 rats in each group).Myocardial I/R was established by ligation of left coronary artery for 30 min,followed by reperfusion for 24 h in I/R group,while not in control group.The myocardial tissue and blood samples were collected to detect the levels of ROS [MDA,MPO and GSH] and RNS [iNOS activity,NOx and ONOO-] and then compared among groups.Results The plasma levels of MDA and MPO were significantly higher,the level of GSH was significantly lower in elderly group than those in adult group 24 h and 3 d after PCI [MDA:(8.6 ±2.3) μmol/L vs (4.8 ± 1.0) μmol/L,(7.5 ± 1.8) μmol/L vs (3.5 ±0.8) μmol/L;MPO:(707 ±224) μg/L vs (474 ± 126) μg/L,(667 ± 187) μg/L vs (377 ± 114) μg/L;GSH:(2.6 ± 1.1) μmol/L vs(3.7 ± 1.0) μmol/L,(1.7 ± 0.9) μmol/L vs (2.5 ± 1.1) μmol/L] (P ＜0.01).The activity of iNOS was significantly decreased in elderly group compared with adult group [(28 ± 11) pmol/mg protein · min vs (34 ± 9) pmol/mg protein · min,(20 ± 8) pmol/mg protein · min vs (24 ± 8) pmol/mg protein · min] (P ＜0.01).The rat experiment data were consistent and supported human study data.Conclusion Aging augments ROS and RNS in humans and rats in vivo,which exacerbates myocardium I/R injury.
Keywords：AgingReactive oxygen speciesReactive nitrogen speciesNitrotyrosine
Publication Date：2016-03-30
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)
Pages：5( 324-328 )