Effect of hepatocyte growth factor mediated by attenuated salmonella on gastric ulcer in rats
Abstract：Objective To explore the effect of hepatocyte growth factor (HGF) mediated by attenuated salmonella on gastric ulcer iu rats.Methods The eukaryotic expression vector of HGF (pcKH) was constructed,then pcKH or control plasmid pcK was electrotransfected into reconbinant attenuated salmonella Ty21a to constructed stain carrying pcKH (named as TPH strain) or pcK (named as TP strain).Totally 40 Wistar rats were randomly divided into normal group, model group, TP group and TPH group (n =10 in each group).Acetic acid-induced gastric ulcer was established in model group, TP group and TPH group, and was respectively given intragastric administration of TP (1 × 109 cfu, dissolved in 10％ sodium bicarbonate) , TPH (1 × 109 efu, dissolved in 10％ sodium bicarbonate) and 10％ sodium bicarbonate on the 5th after injection, 1 time per 3 days,for 3 times.The ulcer healing was observed on gross or microscopic, the ulcer area and crawl length of gastric epithelium were measured;the HGF expression in ulcer tissue was assessed.Results The recombinant attenuated salmonella stain carrying HGF expression vector was successfully constructed.After treatment, the pathology results showed that the gastric epithelium were obviously proliferated, the glands in proliferative gastric epithelium were obviously expanded and the ulcer was filled by granulation tissue with abundant capillary in TPH group;the ulcer healing was not obvious in model group and TP group.The ulcer area in TPH group was significantly smaller than that in model group and TP group [(11 ± 3) mm2 vs (56 ± 6), (43 ± 4) mm2] (P ＜ 0.05);the crawling length of gastric epithelium in TPH group was significantly longer than that in model group and TP group [(879 ±8) mm v s (821 ± 10), (817 ± 14) mm] (P ＜ 0.05).The expression of HGF proteins in gastric ulcer tissue was significantly higher in TPH group than that in model group and TP group [(0.88 ± 0.18) μg/g protein vs (0.51 ±0.08), (0.34±0.15) μg/g protein] (P ＜0.05).Conclusion Recombinant attenuated salmonella stain carrying HGF gene can significantly accelerate the healing of gastric ulcers via stimulating proliferation of gastric epithelial cells and angiogenesis of gastric ulcer wound.
Keywords：Hepatocyte growth factorGene therapyAttenuated salmonellaGastric ulcer
Publication Date：2015-01-01
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)
Pages：5( 1778-1782 )