Effect of dexmedetomidine on lung injury induced by renal ischemia reperfusion in rats

Cui Yan; Li Weijing; Du Wenmin; Ouyang Bishan

doi:10.3760/cma.j.issn.1673-4777.2015.09.014

Abstract：Objective To investigate the effect of dexmedetomidine on lung injury induced by renal ischemia reperfusion in rats.Methods Forty-eight Sprague Dawley rats were randomly divided into control group,lipopolysaccharide (LPS) group and dexmedetomidine group (each n =16).After establishment of lung injury model induced by renal ischemia reperfusion,control group was injected with 0.9％ sodium chloride injection (0.5 ml/kg) ; LPS group was slowly injected with LPS (4 mg/kg) ; dexmedetomidine group was injected with dexmedetomidine (1.0 μg/kg) within 10 min.The behaviors of rats and the pathological changes of lung tissue were observed; the whole lung weight,lung coefficient [organ wet weight (g)/body weight (g)],apoptosis index of lung tissue,tumor necrosis factor α (TNF-α) and interleukin (IL)-6 in lung tissue were measured.Results In dexmedetomidine group,the rats showed good mind and diet,ideal growth and weight gain; pathology results showed that the cell infiltration and destruction of the lung tissue were not obvious.The whole lung weight,lung coefficient,apoptosis index of lung tissue,TNF-α and IL-6 in lung tissue in dexmedetomidine group were all significantly lower than those in LPS group and control group [(1.4 ± 0.4) g vs (2.1 ± 0.4),(2.2 ± 0.4) g ; (7.7 ±0.4)％ vs (10.3 ±0.7)％,(11.7 ±0.5)％ ; (22.1 ±4.4)％ vs (24.9 ±5.1)％,(55.0 ±6.3)％ ; (234 ±74) ng/L vs (400 ±56),(410 ±54) ng/L; (33 ± 19) ng/L vs (67 ± 16),(72 ± 12) ng/L] (P ＜ 0.05) ; all indexes except apoptosis index of lung tissue showed no significant differences between LPS group and control group (P ＞ 0.05).Conclusion Dexmedetomidine can effectively reduce IL-6 and TNF-α expression,relieve the lung inflammation and apoptosis in the lung tissue,which shows a protective effect on lung injury induced by renal ischemia-reperfusion.