Influence of dexmedetomidine on early release of inflammatory cytokines in rat models of cardiopulmonary resuscitation
Li Jiebin
Du Qingxia
Ding Ning
Wang Yu
Abstract：Objective To investigate the influence of dexmedetomidine (DEX) on early release of inflammatory cytokines in rat models of cardiopulmonary resuscitation (CPR).Methods Thirty-six male SD rats were randonly divided into sham operation group,CPR group and DEX group.The rats in sham operation group only underwent anesthesia,endotracheal intubation and vascular puncture without asphyxia and CPR; the cardiac arrest model were established by using asphyxia method,then CPR was done in CPR group and DEX group; DEX group was injected with 50 μg/kg dexmedetomidine and CPR group was injected with the same volume 0.9％ sodium chloride immediately after recovery of spontaneous circulation (ROSC).The levels of serum tumor necrosis factor-α (TNF-α),interleukin-1β (IL-1β),intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) were detected before CPR,3,12 and 24 h after ROSC by using enzyme-linked immunoabsorbent assay.Results All the rats in sham operation group survived for 24 hours ; in CPR group,11 rats recovered spontaneous circulation after CPR and 9 of them survived for 24 hours; in DEX group,10 rats recovered spontaneous circulation after CPR and all survived for 24 hours.Baseline levels of TNF-α,IL-1β,ICAM-l and VCAM-1 were not significantly different among the three groups (P ＞ 0.05).The levels of TNF-α,IL-1β,ICAM-l and VCAM-1 were all increased gradually in CPR group and DEX group after CPR; the levels of TNF-α,IL-1β were significantly lower in DEX group than those in CPR group 3,12 and 24 h after ROSC[TNF-α,3 h:(23 ±5) ng/L vs (40 ± 6) ng/L,12h:(39±9) ng/L vs (84±10) ng/L,24h:(49±9) ng/L vs (92±9) ng/L; IL-1β,3 h:(65± 24) ng/L vs (101 ±24) ng/L,12 h:(95 ±25) ng/L vs (162 ±46) ng/L,24 h:(120 ±42) ng/L vs (212 ± 42) ng/L] (all P ＜ 0.05) ; the levels of ICAM-l and VCAM-1 were significantly lower in DEX group than those in CPR group 24 h after ROSC [(7.9 ±2.3) μg/L vs (18.6 ±6.3) μg/L,(9.9±3.7) μg/L vs (20.5 ± 7.3) μg/L] (both P ＜0.05).Conclusion DEX can significantly inhibit early release of inflammatory cytokines after cardiopulmonary resuscitation,which may be one of the mechanisms for relieving systemic inflammatory reaction.
Keywords：Cardiopulmonary resuscitationDexmedetomidineInflammatory cytokineRat
Publication Date：2015-09-30
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)
Pages：4( 1278-1281 )