Influence of tanshinone Ⅱ A on expression of aquaporin-4 and cerebral edema after cardiopulmonary resuscitationin rats
Zhang Jie
Luo Xurui
Chen Gengjing
Zhang Huili
Abstract：Objective To investigate the influence of tanshinone Ⅱ A on the expression of aquaporin-4 (AQP-4) and cerebral edema after cardiopulmonary resuscitationin rats.Methods Totally 78 Sprague Dawley male rats were randomly divided into sham group(n =6),control group (n =36) and tanshinone Ⅱ A intervention group (n =36).Cardiac arrest was induced by choking models in control group (n =36) and tanshinone Ⅱ A intervention group;tanshinone Ⅱ A intervention group received intravenous injection of tanshinone Ⅱ A (15 mg/kg) and control group was injected with the same amount of 0.9％ sodium chloride injection at recovery instantly.The expression of AQP4 in brain tissue was measured by Immune-histochemical staining at 1,6,12,24,48 and 72 h after restoration of spontaneous circulation (ROSC);the water content of the brain was measured by wet/dry weight method.Results The expression of AQP-4 in control group at 1,6,12,24,48,72 h after ROSC was 10.2 ± 1.9,13.4 ±2.1,15.1 ±2.0,20.5 ±3.0,17.6 ±2.9,14.5 ±2.2,respectively,all significantly higher than that in sham group (7.3 ± 1.2) (P ＜ 0.01);the expression of AQP-4 in tanshinone Ⅱ A intervention group at all time points was 9.8 ± 1.2,11.0 ± 1.9,14.3 ± 2.2,17.1 ± 2.5,16.1 ± 1.7,13.6 ± 2.2 respectively,all significantly lower than that in control group (P ＜ 0.01 or P ＜ 0.05).The water content of brain tissue in control group at 1,6,12,24,48,72 h after ROSC was (78.22 ±0.23)％,(78.39 ± 0.16)％,(79.11 ±0.24)％,(79.43 ±0.20)％,(78.67 ±0.21)％,(78.06 ±0.20)％,respectively,all significantly higher than that in sham group [(77.28 ± 0.09)％] (P ＜ 0.01);the water content of brain tissue intanshinone Ⅱ A intervention group was (77.60 ±0.16)％,(77.96 ±0.21)％,(78.17 ±0.21)％,(78.77 ± 0.28) ％,(78.05 ± 0.23) ％,(77.61 ± 0.23) ％,respectively,all significantly lower than that in control group (P ＜ 0.01 or P ＜ 0.05).Conclusion Tanshinone Ⅱ A exert can reduce the expression of AQP-4 in the brain and relief thebrain edema in rats after cardiopulmonary resuscitation.
Keywords：Brain edemaTanshinone Ⅱ AAquaporin 4Sudden cardiac arrestCardiopulmonary resuscitation
Publication Date：2015-01-01
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)
Pages：4( 1133-1136 )