Effect of apigenin on regulating plasma lipid and improving antioxidant ability in diabetes model induced by streptozocin in rats
Liu Shuang
Cui Weizheng
Abstract：Objective To investigate the effect of apigenin (APG) on regulating plasma lipid and improving antioxidant ability in diabetes model induced by streptozocin (STZ) in rats.Methods One hundred and twenty male Wistar rats were randomly divided into normal control group,diabetes model control group and APG (5,10,20 and 40 mg/kg) treated groups (each n =20).Except for normal control group,the diabetes model were induced by intraperitoneal injection of STZ.The rats in APG (5,10,20 and 40 mg/kg) treated groups were treated with intraperitoneal injection of APG for 4 weeks after establishment of the diabetes model,the rats in normal control group and diabetes model control group were given equal volume of 0.9％ sodium chloride.After 4 weeks of treatment,the levels of total cholesterol (TC),triglyceride (TG),low density lipoprotein cholesterol (LDL-C),high density lipoprotein cholesterol (HDL-C),aspartate aminotransferase (AST),creatine kinase (CK),lactate dehydrogenase (LDH),malonaldehyde (MDA),total antioxidant capacity (T-AOC) in the serum were measured;the activity of superoxide dismutase (SOD) and catalase (CAT),the content of MDA in the myocardial tissue were determined;the histopathological changes of myocardial tissue were observed.Results Compared with diabetes model control group,the levels of TC,TG,LDL-C were significantly decreased in the serum in 20 and 40 mg/kg APG treated groups [TC:(2.7 ±0.7),(2.5 ±0.8) mmol/L vs (3.4± 0.8) mmol/L,TG:(1.23±0.35),(1.10±0.26) mmol/L vs (1.63 ±0.66) mmol/L,LDL-C:(1.04± 0.36),(0.98 ± 0.38) mmol/L vs (1.22 ± 0.38) mmol/L];the level of HDL-C in the serum was significantly increased in 40 mg/kg APG treated group [(0.77 ± 0.29) mmol/L vs (0.61 ± 0.17) mmol/L];the level AST,CK,LDH,MDA in the serum were significantly decreased in 10,20 and 40 mg/kg APG treated groups [AST:(106±31),(99 ±27),(94 ±28) U/L vs (130 ±42) U/L,CK:(862± 186),(837±176),(803± 164) U/L vs (954 ± 225) U/L,LDH:(938 ± 168),(903 ± 149),(865 ± 152) U/L vs (1 133 ± 207) U/L,MDA:(12.7 ±2.8),(12.0 ±2.9),(11.6 ±2.6) nmol/mg vs (14.1 ±3.6) nmol/mg],the T-AOC in the serum were significantly increased in 20 and 40 mg/kg APG treated groups [(10.4 ± 3.1),(11.8 ± 3.2) U/L vs (8.1 ±2.3) U/L] (P ＜0.05 or P ＜0.01).Compared with diabetes model control group,the activity of SOD and CAT in the myocardial tissue were significantly increased,and the content of MDA in the myocardial tissue were significantly decreased in 20 and 40 mg/kg APG treated groups [SOD:(10.2 ± 2.2),(10.9 ± 2.4) U/mg vs (8.9±1.9) U/rmg,CAT:(3.4±0.9),(3.6±1.1) U/mg vs (2.7 ±0.8) U/mg,MDA:(5.5 ± 1.6),(5.2 ± 1.7) nmol/mg vs (6.4 ± 1.8) nmol/mg] (P ＜ 0.05 or P ＜ 0.0l).In diabetes model control group,the myocardial fibers were disordered and fractured,the membrane of myocardial cell was destructed,swelling and vacuoles degeneration were also prominent,which were all improved in 20 and 40 mg/kg APG treated groups.Conclusion APG can effectively improve antioxidant ability,reduce the damage of free radical,improve the cardiac function,and reduce the blood lipid in diabetic rats induced by STZ.
Keywords：ApigeninDiabetesRegulating plasma lipidAntioxidant
Publication Date：2015-01-01
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)
Pages：5( 985-989 )