Effect of ageing on biological function of cardiac stem cells under hypoxic stress
Pan Yu
Xiao Jing
Li Xiaohong
Cai Sa
Yu Xiyong
Abstract：Objective To investigate the effect of ageing on biological function of cardiac stem cells (CSC) under stress of hypoxia.Methods Primitive mouse Sca1 + CSC were isolated and SA-β-Gal staining was used to confirm the ageing features of the 6th passage (P6) Sca1 + CSC.Before and after hypoxic treatment,the proliferation abilities,the mRNA levels of vascular endothelial growth factor (VEGF) and platelet-derived growth factor (PDGF)-A/B,the secretive levels of VEGF and PDGF-BB in P1 and P6 Sca1 + CSC were detected and compared.Ischemic models were established by ligation of the coronary artery in 6 young mice and 6 old mice (ischemic group);in addition,6 young mice and 6 old mice were set as sham group.Three days after establishment of ischemic model,the Sca1 + CSC pool in heart was detected through flow cytometry.Results P6 Sca1 + CSC were positively stained by SA-β-Gal with larger volume compared with P1 Sca1 + CSC,while no positive staining was observed in P6 Sca1 + CSC.The proliferation rate of P6 Sca1 + CSC was significantly lower than that of P1 Sca1 + CSC [(18 ± 5)％ vs (103 ± 27)％,P ＜ 0.01)];after hypoxic treatment,the proliferation rate of P1 Sca1 + CSC [(58 ± 11) ％] was significantly reduced (P ＜ 0.05);no obvious change was found in P6 Sca1 + CSC [(26 ± 8) ％] (P ＞ 0.05).The mRNA levels of VEGF,PDGF-A and PDGF-B were significantly increased in P1 Sca1 + CSC after hypoxic treatment,while no significant changes were found in P6 Sca1 + CSC.After hypoxic treatment,the secretive VEGF and PDGF-BB were significantly increased in P1 Sca1 + CSC [(150 ± 37) μg/L vs (18 ± 6) μg/L,(288 ± 37) μg/L vs (35 ± 6) μg/L,P ＜ 0.01],while no significant changes were found in P6 Sca1 + CSC (P ＞ 0.05).In experiment in vivo,the population of Sca1 + CSC in ischemic group was significantly larger than that in sham group [old mice:(13.2 ± 4.2) ％ vs (1.5 ± 0.4％) ％,young mice:(31.7 ± 6.5) ％ vs (5.0 ±0.6)％];the population of Sca1 + CSC in old mice was significantly smaller than that in young mice in both groups (P ＜ 0.05).Conclusion In Sca1 + CSC,the proliferation ability,secretion and mobilization in response to the hypoxic stress are reduced due to ageing.
Keywords：AgeingCardiac stem cellsHypoxia
Publication Date：2015-01-01
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)
Pages：5( 933-937 )