Expression of connexin 43 in non-infarction related coronary arteries and stimulation mechanism of angiotensin Ⅱ in animal model of acute ST elevation myocardial infarction
Abstract：Objective To explore the expression of connexin 43 (Cx43) in non-infarction related coronary arteries and the mechanism of angiotensin Ⅱ] (Ang]Ⅱ) in stimulating the expression of Cx43 in animal model of acute ST elevation myocardial infarction.Methods The hyperlipidemia model was prepared in rabbits and 8 of them were selected to establish the acute ST elevation myocardial ischemia reperfusion model (myocardial ischemia-reperfusion group) and another 8 of them were set as control group.One week after establishment of myocardial ischemia-reperfusion model,the fluorescence immunohistochemistry method was used to semiquantitatively measure the expression of Cx43 in non-infarction related arteries.The smooth muscle cells in non-infarction related arteries were isolated from normal and hyperlipidemia rabbits and were treated with different methods.Normal control group (12 sample):normal smooth muscle cells + phosphate buffer (PBS);hyperlipidemia control group (12 sample):hyperlipidemia smooth muscle cells + PBS;Ang Ⅱ intervention group (12 sample):hyperlipidemia smooth muscle cells + Ang Ⅱ (0.1 μmol/L,24 h);mitogen activated protein kinase (MAPK) signal transduction pathway inhibitor pretreatment + Ang Ⅱ intervention groups (12 sample):hyperlipidemia smooth muscle cells + PD98059 (100 μmol/L,30 min) or SP600125 (25 μmol/L,30 min) or SB203580 (25 μmol/L,30 min) + Ang Ⅱ (0.1 μmol/L,24 h).The expression of Cx43 was analyzed and compared among different groups.Results Atherosclerotic plaques were found in non-infarction related arteries in myocardial ischemiareperfusion group,and the expression of Cx43 in non-infarction related arteries was significantly increased compared with that in control group (absorbance value:2 136 ±238 vs 1 303 ± 142,P ＜0.05).The expression of Cx43 in Ang Ⅱ intervention group was significantly higher than that in hyperlipidemia control group and normal control group [(1.79 ±0.31) vs (1.25±0.21),(0.62 ±0.14),P＜0.05)];the expression of Cx43 in MAPK signal transduction pathway inhibitor pretreatment ± Ang Ⅱ intervention groups was all significantly lower than that in Ang Ⅱ] intervention group [(0.85 ± 0.19),(0.99 ± 0.13),(1.79 ± 0.31),(0.81 ± 0.15) vs (1.79 ± 0.31),P ＜ 0.05)].Conclusions Ang Ⅱ can stimulate the expression of Cx43 in non-infarction related arteries in acute ST elevation myocardial infarction through MAPK signal pathway.
Keywords：Non infarction related arteriesConnexin 43Angiotensin Ⅱ
Publication Date：2015-01-01
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)
Pages：4( 793-796 )