Effect of esomeprazole combined with irsogladine in treatment of small intestine injury induced by non steroidal anti inflammatory drug
Zou Jianping
Zhang Jianjun
Ma Xiaoyan
Wang Zhiheng
Wang Shufeng
Abstract：Objective To explore the effect of esomeprazole combined with irsogladine on small intestine injury induced by non steroidal anti inflammatory drug (NSAID).Methods Fifty adult SD rats were randomly divided into control group,model group,irsogladine maleate group,esomeprazole group and irsogladine maleate combined with esomeprazole group (each n =10).Model group and the three drug groups were administrated with 15 mg/kg diclofenac and control group was administrated with 0.9％ sodium chloride (intragastric administration) for 3 days,and then the related drugs were administrated the three drug groups,and 0.9％ sodium chloride were administrated in control group and model group for 7 days.After 7 days of treatment,the animals were sacrificed,the pathological changes of the small intestine were observed using hematoxylin-eosin stain and the gross morphological and histological injury were scored.The quantitative polymerase chain reaction was used to detect the mRNA level of cyclooxygenase (COX)-1 and COX-2.Results The gross morphological and histopathological injury score of the small intestine were obviously higher in model group compared with those in control group [(4.59 ± 0.40) scores vs (0.00 ± 0.00) scores,(4.00 ± 0.08) scores vs (0.00 ± 0.00) scores] (P ＜ 0.05),while were obviously lower in irsogladine maleate group,esomeprazole group and irsogladine maleate combined with esomeprazole group compared with in model group [(2.80 ± 1.21),(3.10 ± 1.02),(1.98 ± 0.88) scores vs (4.59 ±0.40) scores,(2.21 ±1.01),(2.18 ±0.91),(1.67 ±0.98) scores vs (4.00 ±0.08) scores] (P＜ 0.05),with statistical differences between irsogladine maleate combined with esomeprazole group and irsogladine maleate group,esomeprazole group (P ＜ 0.05).The COX-1 mRNA was significantly lower and the COX-2 mRNA was significantly higher in model group compared with control group (1.09 ± 0.40 vs 3.23 ± 0.65,3.50 ± 0.34 vs 0.23 ± 0.12)(P ＜ 0.05),while were both improved in irsogladine maleate group,esomeprazole group and irsogladine maleate combined with esomeprazole group (3.40 ± 1.32,3.28 ± 1.42,4.98 ± 0.88 vs 1.09 ± 0.40;2.21 ±1.01,2.37 ±0.78,2.01 ±0.98 vs 3.50 ±0.34) (P ＜0.05).There were significant differences in COX-1 mRNA (P ＜ 0.05) and no significant differences in COX-2 mRNA (P ＞ 0.05) between irsogladine maleate combined with esomeprazole group and irsogladine maleate group.Conclusion Irsogladine maleate combined with esomeprazole is more effective in prevention of NSAID induced mucosa injury of small intestine compared with single drug application.
Keywords：Small intestinal injuryNon-steroidal anti-inflammatory drugEsomeprazoleIrsogladine maleate
Publication Date：2015-01-01
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)
Pages：5( 671-675 )