The influence of oxcarbazepine and carbamazepine on cognitive function of epileptic rats
Sun Ran
Wang Yanling
Li Lei
Chu Xu
Xia Min
Kong Qingxia
Abstract：Objective To study the expression changes of neural celladhesion molecule 1 (NCAM 1) and extracellular signalregulated kinases 2 (ERK-2) in a rat model of epilepsy'hippocampus with cognitive dysfunction.Methods Male Wistar rats were randomly divided into control group,epilepsy group,carbamazepine group and oxcarbazepine group(20 cases in each group).In expermental group,lithium chloride and methylscopolamine were uesd to build the rat model of epilepsy.The control model group had saline.The carbamazepine and oxcarbazepine were respective used in different groups as treatment(i.g,200mg/d per rat).The learning and memory ability was observed.The Hippocampus was dissected one day after this text.RT-PCR was used to detect the mRNA expression of NCAM-1 and ERK-2.ELISA was used to detect the protein expression of NCAM 1and ERK-2.Results The results of mean escape latency of Morris water maze test showed that carbamazepine group was (81 ±9)s and (27 ±9)s ;oxcarbazepine group was (72 ±9)s and (32 ± 14) s;epilepsy group was (67 ±7)s and (37 ± 13)s;control group was (36 ±5)s and (51 ± 11)s(P ＜0.01) The expression level of NCAM/β-actin and ERK-2/β3-actin was 0.60 ± 0.12 and 0.43 ± 0.11 (carbamazepine group) ; 0.66 ± 0.03 and 0.51 ± 0.17 (oxcarbazepine group) ;0.95 ± 0.21 and 0.59 ± 0.24 (epilepsy group) ; 0.48 ± 0.04 and 0.75 ± 0.23 (control group).The gene expression level of NCAM-1 was lower in the treatment group compared with that in epilepsy group(P ＜0.01).The expression level of ERK-2 was lower in the treatment groups compared with that in the epilepsy group.Conclusions The expression of ERK-2 in the hippocampus was decreased and NCAM-1 was increased at 1 month.The results showed thatboth carbamazepine and oxcarbazepin participated in the pathogenesy of cognitive dysfunctionof epilepsy.Carbamazepine can aggratate the cognitive dysfunction.
Keywords：EpilepsyNeural celladhesion molecule-1Extracellular signal-regulated kinases 2OxcarbazepineCognitive function
Publication Date：2015-01-30
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)
Pages：5( 77-81 )