Experimental study on the reversal effect of bortezomib on human acute lymphocytic leukemia cell lines
Du Xiaoli
Yang Min
Chen Qi
Abstract：Objective To investigate the possible mechanisms how bortezomib inhibits the proliferation of human acute lymphocytic leukemia cell lines.Methods The Nalm-6 cells were devided into control group cultured with RPMI 1640 medium and durg group cultured with RPMI 1640 medium plus different final density of bortezomib(100,200,400,800,1 600 nmol/L).Different density and different action time of bortezomib were measured to investigate the impact on the growth suppression of Nalm-6 cell.The impact of 400 nmol/L bortezomib on the cell cycle was measured by flow cytometry assay.Results ①After Nalm-6 cell in vitro culture with 100,200,400,800,1 600 nmol/L bortezomib for 12 h,the rate of Nalm-6 cell growth suppression was(8.0 ± 6.1)％,(18.3±7.5)％,(30.3±7.5)％,(37.8 ±2.6)％,(41.1 ±2.1)％ respectively;(13.5±5.2)％,(43.3± 6.5)％,(68.7±2.1)％,(81.6±1.6)％,(87.4 ±1.4)％ after24 h; (5.21 ±3.71)％,(9.33 ±2.53)％,(49.32±7.12)％,(97.71±1.13)％,(99.93±0.11)％ after48 h;(4.00±3.62)％,(4.32±5.61)％,(8.32 ± 4.42) ％,(79.52 ± 8.61) ％,(99.94 ± 0.13) ％ after 72 h.The different rate had statistical significance between experimental group and control group(P ＜ 0.05).②After 400 nmol/L bortezomib,cell cycle G2/M phase ratio was(18.6 ± 2.4)％ and it was (3.8 ± 2.1)％ in the control group; the difference had statistical significance (P ＜ 0.05).Conclusions Bortezomib can inhibit the proliferation of Nalm-6 cell.Bortezomib can block the cell cycle of Nalm-6 cell at the stage of G2/M.
Keywords：Acute lymphoblastic leukemiaBortezomibCell cycle
Publication Date：2015-01-01
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)
Pages：3( 31-33 )